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7O6H

Crystal structure of Paradendryphiella salina PL7A alginate lyase mutant Y223F in complex with tri-mannuronic acid

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsMAX IV BEAMLINE BioMAX
Synchrotron siteMAX IV
BeamlineBioMAX
Temperature [K]100
Detector technologyPIXEL
Collection date2020-02-20
DetectorDECTRIS EIGER X 16M
Wavelength(s)0.972425
Spacegroup nameP 21 21 21
Unit cell lengths34.980, 81.220, 80.800
Unit cell angles90.00, 90.00, 90.00
Refinement procedure
Resolution36.280 - 1.660
R-factor0.1759
Rwork0.174
R-free0.20360
Structure solution methodMOLECULAR REPLACEMENT
Starting model (for MR)7ncz
RMSD bond length0.008
RMSD bond angle1.041
Data reduction softwareXDS
Data scaling softwareXDS
Phasing softwarePHASER (1.18.2_3874)
Refinement softwarePHENIX (1.18.2_3874)
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]40.6101.760
High resolution limit [Å]1.6601.660
Number of reflections279534424
<I/σ(I)>14.16
Completeness [%]99.9
Redundancy7.34
CC(1/2)1.0000.580
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, SITTING DROP429320% PEG 1500 and 0.1 M TBG buffer pH 4

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PDB entries from 2024-11-06

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