7O4K
Yeast TFIIH in the contracted state within the pre-initiation complex
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (0) | General transcription and DNA repair factor IIH helicase subunit XPD | polymer | 778 | 89899.0 | 1 | UniProt (P06839) Pfam (PF06733) Pfam (PF06777) Pfam (PF13307) | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) | TFIIH subunit XPD,DNA repair helicase RAD3,RNA polymerase II transcription factor B subunit RAD3,TFB subunit RAD3 |
| 2 | B (1) | General transcription and DNA repair factor IIH subunit TFB1 | polymer | 645 | 73194.5 | 1 | UniProt (P32776) Pfam (PF08567) Pfam (PF03909) | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) | TFIIH subunit TFB1,RNA polymerase II transcription factor B 73 kDa subunit,RNA polymerase II transcription factor B p73 subunit,RNA polymerase II transcription factor B subunit 1 |
| 3 | C (2) | General transcription and DNA repair factor IIH subunit TFB2 | polymer | 517 | 58900.6 | 1 | UniProt (Q02939) Pfam (PF03849) Pfam (PF18307) | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) | TFIIH subunit TFB2,RNA polymerase II transcription factor B 52 kDa subunit,RNA polymerase II transcription factor B p52 subunit,RNA polymerase II transcription factor B subunit 2 |
| 4 | D (3) | RNA polymerase II transcription factor B subunit 3 | polymer | 324 | 38480.7 | 1 | UniProt (Q03290) Pfam (PF06391) Pfam (PF17121) | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) | RNA polymerase II transcription factor B 38 kDa subunit,RNA polymerase II transcription factor B p38 subunit |
| 5 | E (4) | General transcription and DNA repair factor IIH subunit TFB4 | polymer | 341 | 37778.7 | 1 | UniProt (Q12004) Pfam (PF03850) | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) | TFIIH subunit TFB4,RNA polymerase II transcription factor B 34 kDa subunit,RNA polymerase II transcription factor B p34 subunit,RNA polymerase II transcription factor B subunit 4 |
| 6 | F (5) | General transcription and DNA repair factor IIH subunit TFB5 | polymer | 76 | 8541.8 | 1 | UniProt (Q3E7C1) Pfam (PF06331) | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) | TFIIH subunit TFB5,RNA polymerase II transcription factor B subunit 5 |
| 7 | G (6) | General transcription and DNA repair factor IIH subunit SSL1 | polymer | 464 | 52571.2 | 1 | UniProt (Q04673) Pfam (PF04056) Pfam (PF07975) | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) | TFIIH subunit SSL1,RNA polymerase II transcription factor B subunit SSL1,TFB subunit SSL1,Suppressor of stem-loop protein 1 |
| 8 | H (7) | General transcription and DNA repair factor IIH helicase subunit XPB | polymer | 843 | 95461.7 | 1 | UniProt (Q00578) Pfam (PF13625) Pfam (PF04851) Pfam (PF16203) | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) | TFIIH subunit XPB,DNA repair helicase RAD25,RNA polymerase II transcription factor B subunit SSL2,TFB subunit SSL2,Suppressor of stem-loop mutation 2 |
| 9 | I (A) | DNA-directed RNA polymerase II subunit RPB1 | polymer | 1733 | 191821.6 | 1 | UniProt (P04050) Pfam (PF04997) Pfam (PF00623) Pfam (PF04983) Pfam (PF05000) Pfam (PF04998) Pfam (PF04992) Pfam (PF04990) Pfam (PF05001) | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) | RNA polymerase II subunit B1,DNA-directed RNA polymerase III largest subunit,RNA polymerase II subunit B220 |
| 10 | J (B) | DNA-directed RNA polymerase II subunit RPB2 | polymer | 1224 | 138937.3 | 1 | UniProt (P08518) Pfam (PF04563) Pfam (PF04561) Pfam (PF04565) Pfam (PF04566) Pfam (PF04567) Pfam (PF00562) Pfam (PF04560) | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) | RNA polymerase II subunit 2,B150,DNA-directed RNA polymerase II 140 kDa polypeptide |
| 11 | K (D) | DNA-directed RNA polymerase II subunit RPB4 | polymer | 221 | 25451.2 | 1 | UniProt (P20433) Pfam (PF03874) | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) | RNA polymerase II subunit B4,B32,DNA-directed RNA polymerase II 32 kDa polypeptide |
| 12 | L (F) | DNA-directed RNA polymerases I, II, and III subunit RPABC2 | polymer | 155 | 17931.8 | 1 | UniProt (P20435) Pfam (PF01192) | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) | RNA polymerases I,II,and III subunit ABC2,ABC23,DNA-directed RNA polymerases I,and III 23 kDa polypeptide |
| 13 | M (G) | DNA-directed RNA polymerase II subunit RPB7 | polymer | 177 | 19909.9 | 1 | UniProt (P34087) Pfam (PF03876) Pfam (PF00575) | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) | RNA polymerase II subunit B7,B16 |
| 14 | N (N) | Non-template DNA | polymer | 106 | 32717.0 | 1 | synthetic construct | ||
| 15 | O (T) | Template DNA | polymer | 106 | 32680.9 | 1 | synthetic construct | ||
| 16 | P (W) | Transcription initiation factor IIE subunit alpha | polymer | 492 | 55951.0 | 1 | UniProt (P36100) Pfam (PF02002) | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) | TFIIE-alpha,Factor A 66 kDa subunit,Transcription factor A large subunit |
| 17 | Q (X) | Transcription initiation factor IIE subunit beta | polymer | 328 | 37050.4 | 1 | UniProt (P36145) Pfam (PF02186) Pfam (PF18121) Pfam (PF22254) | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) | TFIIE-beta,Factor A 43 kDa subunit,Transcription factor A small subunit |
| 18 | R (0) | IRON/SULFUR CLUSTER | non-polymer | 351.6 | 1 | Chemie (SF4) | |||
| 19 | CA, DA, EA, S, T... (A, B, W, 3, 4...) | ZINC ION | non-polymer | 65.4 | 10 | Chemie (ZN) | |||
| 20 | Z (7) | BERYLLIUM TRIFLUORIDE ION | non-polymer | 66.0 | 1 | Chemie (BEF) | |||
| 21 | AA (7) | MAGNESIUM ION | non-polymer | 24.3 | 1 | Chemie (MG) | |||
| 22 | BA (7) | ADENOSINE-5'-DIPHOSPHATE | non-polymer | 427.2 | 1 | Chemie (ADP) |
Sequence modifications
1: 1 - 642 (UniProt: P32776)
2: 1 - 513 (UniProt: Q02939)
3: 1 - 321 (UniProt: Q03290)
4: 1 - 338 (UniProt: Q12004)
5: 1 - 72 (UniProt: Q3E7C1)
6: 1 - 461 (UniProt: Q04673)
G: 1 - 171 (UniProt: P34087)
W: 1 - 482 (UniProt: P36100)
| PDB | External Database | Details |
|---|---|---|
| Gly -2 | - | expression tag |
| Gly -1 | - | expression tag |
| Ser 0 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Gly -3 | - | expression tag |
| Pro -2 | - | expression tag |
| Gly -1 | - | expression tag |
| Ser 0 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Gly -2 | - | expression tag |
| Pro -1 | - | expression tag |
| His 0 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Ser -2 | - | expression tag |
| Asn -1 | - | expression tag |
| Ala 0 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Gly -3 | - | expression tag |
| Pro -2 | - | expression tag |
| Gly -1 | - | expression tag |
| Ser 0 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Gly -2 | - | expression tag |
| Gly -1 | - | expression tag |
| Ser 0 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| His 172 | - | expression tag |
| His 173 | - | expression tag |
| His 174 | - | expression tag |
| His 175 | - | expression tag |
| His 176 | - | expression tag |
| His 177 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Ala 483 | - | expression tag |
| Ala 484 | - | expression tag |
| Ala 485 | - | expression tag |
| Leu 486 | - | expression tag |
| Glu 487 | - | expression tag |
| His 488 | - | expression tag |
| His 489 | - | expression tag |
| His 490 | - | expression tag |
| His 491 | - | expression tag |
| His 492 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 17 |
| Total formula weight | 1007279.5 | |
| Non-Polymers* | Number of molecules | 14 |
| Total formula weight | 1523.2 | |
| All* | Total formula weight | 1008802.7 |
