7O4K

Yeast TFIIH in the contracted state within the pre-initiation complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	0	General transcription and DNA repair factor IIH helicase subunit XPD	polymer	778	89899.0	1	UniProt (P06839) Pfam (PF06733) Pfam (PF06777) Pfam (PF13307) In PDB	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)	TFIIH subunit XPD,DNA repair helicase RAD3,RNA polymerase II transcription factor B subunit RAD3,TFB subunit RAD3
2	1	General transcription and DNA repair factor IIH subunit TFB1	polymer	645	73194.5	1	UniProt (P32776) Pfam (PF08567) Pfam (PF03909) In PDB	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)	TFIIH subunit TFB1,RNA polymerase II transcription factor B 73 kDa subunit,RNA polymerase II transcription factor B p73 subunit,RNA polymerase II transcription factor B subunit 1
3	2	General transcription and DNA repair factor IIH subunit TFB2	polymer	517	58900.6	1	UniProt (Q02939) Pfam (PF03849) Pfam (PF18307) In PDB	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)	TFIIH subunit TFB2,RNA polymerase II transcription factor B 52 kDa subunit,RNA polymerase II transcription factor B p52 subunit,RNA polymerase II transcription factor B subunit 2
4	3	RNA polymerase II transcription factor B subunit 3	polymer	324	38480.7	1	UniProt (Q03290) Pfam (PF06391) Pfam (PF17121) In PDB	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)	RNA polymerase II transcription factor B 38 kDa subunit,RNA polymerase II transcription factor B p38 subunit
5	4	General transcription and DNA repair factor IIH subunit TFB4	polymer	341	37778.7	1	UniProt (Q12004) Pfam (PF03850) In PDB	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)	TFIIH subunit TFB4,RNA polymerase II transcription factor B 34 kDa subunit,RNA polymerase II transcription factor B p34 subunit,RNA polymerase II transcription factor B subunit 4
6	5	General transcription and DNA repair factor IIH subunit TFB5	polymer	76	8541.8	1	UniProt (Q3E7C1) Pfam (PF06331) In PDB	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)	TFIIH subunit TFB5,RNA polymerase II transcription factor B subunit 5
7	6	General transcription and DNA repair factor IIH subunit SSL1	polymer	464	52571.2	1	UniProt (Q04673) Pfam (PF04056) Pfam (PF07975) In PDB	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)	TFIIH subunit SSL1,RNA polymerase II transcription factor B subunit SSL1,TFB subunit SSL1,Suppressor of stem-loop protein 1
8	7	General transcription and DNA repair factor IIH helicase subunit XPB	polymer	843	95461.7	1	UniProt (Q00578) Pfam (PF13625) Pfam (PF04851) Pfam (PF16203) In PDB	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)	TFIIH subunit XPB,DNA repair helicase RAD25,RNA polymerase II transcription factor B subunit SSL2,TFB subunit SSL2,Suppressor of stem-loop mutation 2
9	A	DNA-directed RNA polymerase II subunit RPB1	polymer	1733	191821.6	1	UniProt (P04050) Pfam (PF04997) Pfam (PF00623) Pfam (PF04983) Pfam (PF05000) Pfam (PF04998) Pfam (PF04992) Pfam (PF04990) Pfam (PF05001) In PDB	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)	RNA polymerase II subunit B1,DNA-directed RNA polymerase III largest subunit,RNA polymerase II subunit B220
10	B	DNA-directed RNA polymerase II subunit RPB2	polymer	1224	138937.3	1	UniProt (P08518) Pfam (PF04563) Pfam (PF04561) Pfam (PF04565) Pfam (PF04566) Pfam (PF04567) Pfam (PF00562) Pfam (PF04560) In PDB	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)	RNA polymerase II subunit 2,B150,DNA-directed RNA polymerase II 140 kDa polypeptide
11	D	DNA-directed RNA polymerase II subunit RPB4	polymer	221	25451.2	1	UniProt (P20433) Pfam (PF03874) In PDB	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)	RNA polymerase II subunit B4,B32,DNA-directed RNA polymerase II 32 kDa polypeptide
12	F	DNA-directed RNA polymerases I, II, and III subunit RPABC2	polymer	155	17931.8	1	UniProt (P20435) Pfam (PF01192) In PDB	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)	RNA polymerases I,II,and III subunit ABC2,ABC23,DNA-directed RNA polymerases I,and III 23 kDa polypeptide
13	G	DNA-directed RNA polymerase II subunit RPB7	polymer	177	19909.9	1	UniProt (P34087) Pfam (PF03876) Pfam (PF00575) In PDB	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)	RNA polymerase II subunit B7,B16
14	N	Non-template DNA	polymer	106	32717.0	1		synthetic construct
15	T	Template DNA	polymer	106	32680.9	1		synthetic construct
16	W	Transcription initiation factor IIE subunit alpha	polymer	492	55951.0	1	UniProt (P36100) Pfam (PF02002) In PDB	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)	TFIIE-alpha,Factor A 66 kDa subunit,Transcription factor A large subunit
17	X	Transcription initiation factor IIE subunit beta	polymer	328	37050.4	1	UniProt (P36145) Pfam (PF02186) Pfam (PF18121) Pfam (PF22254) In PDB	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)	TFIIE-beta,Factor A 43 kDa subunit,Transcription factor A small subunit
18	0	IRON/SULFUR CLUSTER	non-polymer		351.6	1	Chemie (SF4)
19	A, B, W, 3, 4...	ZINC ION	non-polymer		65.4	10	Chemie (ZN)
20	7	BERYLLIUM TRIFLUORIDE ION	non-polymer		66.0	1	Chemie (BEF)
21	7	MAGNESIUM ION	non-polymer		24.3	1	Chemie (MG)
22	7	ADENOSINE-5'-DIPHOSPHATE	non-polymer		427.2	1	Chemie (ADP)

Sequence modifications

1: 1 - 642 (UniProt: P32776)

PDB	External Database	Details
Gly -2	-	expression tag
Gly -1	-	expression tag
Ser 0	-	expression tag

2: 1 - 513 (UniProt: Q02939)

PDB	External Database	Details
Gly -3	-	expression tag
Pro -2	-	expression tag
Gly -1	-	expression tag
Ser 0	-	expression tag

3: 1 - 321 (UniProt: Q03290)

PDB	External Database	Details
Gly -2	-	expression tag
Pro -1	-	expression tag
His 0	-	expression tag

4: 1 - 338 (UniProt: Q12004)

PDB	External Database	Details
Ser -2	-	expression tag
Asn -1	-	expression tag
Ala 0	-	expression tag

5: 1 - 72 (UniProt: Q3E7C1)

PDB	External Database	Details
Gly -3	-	expression tag
Pro -2	-	expression tag
Gly -1	-	expression tag
Ser 0	-	expression tag

6: 1 - 461 (UniProt: Q04673)

PDB	External Database	Details
Gly -2	-	expression tag
Gly -1	-	expression tag
Ser 0	-	expression tag

G: 1 - 171 (UniProt: P34087)

PDB	External Database	Details
His 172	-	expression tag
His 173	-	expression tag
His 174	-	expression tag
His 175	-	expression tag
His 176	-	expression tag
His 177	-	expression tag

W: 1 - 482 (UniProt: P36100)

PDB	External Database	Details
Ala 483	-	expression tag
Ala 484	-	expression tag
Ala 485	-	expression tag
Leu 486	-	expression tag
Glu 487	-	expression tag
His 488	-	expression tag
His 489	-	expression tag
His 490	-	expression tag
His 491	-	expression tag
His 492	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	17
	Total formula weight	1007279.5
Non-Polymers*	Number of molecules	14
	Total formula weight	1523.2
All*	Total formula weight	1008802.7

*Water molecules are not included.