7O1M

Structure of Mycobacterium tuberculosis beta-oxidation trifunctional enzyme alpha-H462A, beta-C92A mutant

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (D)	Putative acyltransferase Rv0859	polymer	403	42428.3	1	UniProt (O53871) Pfam (PF00108) Pfam (PF02803)	Mycobacterium tuberculosis H37Rv
2	B (A)	3-hydroxyacyl-CoA dehydrogenase	polymer	736	77938.7	1	UniProt (O53872) Pfam (PF00378) Pfam (PF02737) Pfam (PF00725)	Mycobacterium tuberculosis H37Rv
3	C, D, E, F, H... (D, A)	SULFATE ION	non-polymer		96.1	7	Chemie (SO4)
4	G (A)	GLYCEROL	non-polymer		92.1	1	Chemie (GOL)
5	K, L (D, A)	water	water		18.0	8	Chemie (HOH)

Sequence modifications

D: 1 - 403 (UniProt: O53871)

PDB	External Database	Details
Ala 92	Cys 92	engineered mutation

A: 1 - 720 (UniProt: O53872)

PDB	External Database	Details
Met -15	-	initiating methionine
Gly -14	-	expression tag
Ser -13	-	expression tag
Ser -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
His -9	-	expression tag
His -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
Ser -5	-	expression tag
Gln -4	-	expression tag
Asp -3	-	expression tag
Pro -2	-	expression tag
Asn -1	-	expression tag
Ser 0	-	expression tag
Ala 462	His 462	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	120367.0
Non-Polymers*	Number of molecules	8
	Total formula weight	764.5
All*	Total formula weight	121131.6

*Water molecules are not included.