7NZM
Cryo-EM structure of pre-dephosphorylation complex of phosphorylated eIF2alpha with trapped holophosphatase (PP1A_D64A/PPP1R15A/G-actin/DNase I)
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (E) | Eukaryotic translation initiation factor 2 subunit 1 | polymer | 186 | 21817.9 | 1 | UniProt (P05198) Pfam (PF00575) Pfam (PF07541) | Homo sapiens (Human) | Eukaryotic translation initiation factor 2 subunit alpha,eIF-2-alpha,eIF-2A,eIF-2alpha |
| 2 | B (B) | Serine/threonine-protein phosphatase PP1-alpha catalytic subunit | polymer | 294 | 33647.6 | 1 | UniProt (P62139) Pfam (PF16891) Pfam (PF00149) | Oryctolagus cuniculus (Rabbit) | PP-1A |
| 3 | C (A) | Actin, alpha skeletal muscle, intermediate form | polymer | 375 | 41862.6 | 1 | UniProt (P68135) Pfam (PF00022) | Oryctolagus cuniculus (Rabbit) | |
| 4 | D (D) | Deoxyribonuclease-1 | polymer | 260 | 29092.6 | 1 | UniProt (P00639) Pfam (PF03372) | Bos taurus (Bovine) | Deoxyribonuclease I,DNase I |
| 5 | E (C) | Protein phosphatase 1 regulatory subunit 15A,Maltose/maltodextrin-binding periplasmic protein | polymer | 444 | 49169.8 | 1 | UniProt (O75807) UniProt (P0AEX9) Pfam (PF10488) Pfam (PF01547) | Homo sapiens (Human) | Growth arrest and DNA damage-inducible protein GADD34,Myeloid differentiation primary response protein MyD116 homolog,MMBP,Maltodextrin-binding protein,Maltose-binding protein,MBP |
| 6 | F (F) | 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 424.4 | 1 | In PDB GlyTouCan (G42666HT) | |||
| 7 | G (B) | MANGANESE (II) ION | non-polymer | 54.9 | 1 | Chemie (MN) | |||
| 8 | H (A) | ADENOSINE-5'-TRIPHOSPHATE | non-polymer | 507.2 | 1 | Chemie (ATP) |
Sequence modifications
B: 7 - 300 (UniProt: P62139)
C: 553 - 624 (UniProt: O75807)
C: 626 - 996 (UniProt: P0AEX9)
| PDB | External Database | Details |
|---|---|---|
| Ala 64 | Asp 64 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| Gln 625 | - | linker |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 5 |
| Total formula weight | 175590.5 | |
| Branched | Number of molecules | 1 |
| Total formula weight | 424.4 | |
| Non-Polymers* | Number of molecules | 2 |
| Total formula weight | 562.1 | |
| All* | Total formula weight | 176577.0 |
