7NY3

Crystal structure of Paradendryphiella salina PL7A alginate lyase mutant Y223F in complex with tetra-mannuronic acid

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Alginate lyase (PL7)	polymer	231	25452.7	1	UniProt (A0A485PVH1) Pfam (PF08787) In PDB	Paradendryphiella salina
2	B	beta-D-mannopyranuronic acid-(1-4)-beta-D-mannopyranuronic acid-(1-4)-beta-D-mannopyranuronic acid-(1-4)-beta-D-mannopyranuronic acid-(1-4)-beta-D-mannopyranuronic acid-(1-4)-beta-D-mannopyranuronic acid	branched		1074.8	1	In PDB GlyTouCan (G52261FN)
3	A	2-[BIS-(2-HYDROXY-ETHYL)-AMINO]-2-HYDROXYMETHYL-PROPANE-1,3-DIOL	non-polymer		209.2	1	Chemie (BTB)
4		water	water		18.0	209	Chemie (HOH)

Sequence modifications

A: 23 - 243 (UniProt: A0A485PVH1)

PDB	External Database	Details
Glu -1	-	expression tag
Phe 0	-	expression tag
Phe 223	Tyr 223	engineered mutation
Val 244	-	expression tag
Asp 245	-	expression tag
His 246	-	expression tag
His 247	-	expression tag
His 248	-	expression tag
His 249	-	expression tag
His 250	-	expression tag
His 251	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	25452.7
Branched	Number of molecules	1
	Total formula weight	1074.8
Non-Polymers*	Number of molecules	1
	Total formula weight	209.2
All*	Total formula weight	26736.7

*Water molecules are not included.