7NNK
Crystal structure of S116A mutant of hydroxy ketone aldolase (SwHKA) from Sphingomonas wittichii RW1 in complex with hydroxypyruvate
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (A, B) | HpcH/HpaI aldolase | polymer | 252 | 26768.5 | 2 | UniProt (A5VH82) UniProt (by SIFTS) (A0AAT8XT72) | Sphingomonas wittichii (strain RW1 / DSM 6014 / JCM 10273) | Hydroxy Ketoacid Aldolase |
| 2 | C (A) | 3-HYDROXYPYRUVIC ACID | non-polymer | 104.1 | 1 | Chemie (3PY) | |||
| 3 | D, E (A, B) | MAGNESIUM ION | non-polymer | 24.3 | 2 | Chemie (MG) | |||
| 4 | F, G (A, B) | water | water | 18.0 | 299 | Chemie (HOH) |
Sequence modifications
A, B: 2 - 251 (UniProt: A5VH82)
| PDB | External Database | Details |
|---|---|---|
| His 0 | - | expression tag |
| His 1 | - | expression tag |
| Ala 116 | Ser 116 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 53537.1 | |
| Non-Polymers* | Number of molecules | 3 |
| Total formula weight | 152.7 | |
| All* | Total formula weight | 53689.7 |
