7NNK

Crystal structure of S116A mutant of hydroxy ketone aldolase (SwHKA) from Sphingomonas wittichii RW1 in complex with hydroxypyruvate

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0003824	molecular_function	catalytic activity
B	0003824	molecular_function	catalytic activity

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	2
Details	Active site: {"description":"Proton acceptor","evidences":[{"source":"UniProtKB","id":"Q47098","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

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site_id	SWS_FT_FI3
Number of Residues	2
Details	Site: {"description":"Transition state stabilizer","evidences":[{"source":"UniProtKB","id":"Q47098","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

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site_id	SWS_FT_FI4
Number of Residues	2
Details	Site: {"description":"Increases basicity of active site His","evidences":[{"source":"UniProtKB","id":"Q47098","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

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site_id	SWS_FT_FI5
Number of Residues	2
Details	Site: {"description":"Improves activity by CH-pi interactions","evidences":[{"source":"Reference","evidenceCode":"ECO:0000305","citation":{"citationType":"journal article","publicationDate":"2019","firstPage":"2649","lastPage":"2649","volume":"361","journal":"Adv. Synth. Catal.","title":"CH-pi Interactions Promote the Conversion of Hydroxypyruvate in a Class II Pyruvate Aldolase.","authors":["Marsden S.R.","Mestrom L.","Bento I.","Hagedoorn P.L.","McMillan D.G.G.","Hanefeld U."],"citationCrossReferences":[{"database":"DOI","id":"10.1002/adsc.201900205"}]}}]}

Chain

Residue

Details

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