7NMI

Transactivation domain of p53 in complex with S100P, using annexin A2 as crystallization chaperone

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Cellular tumor antigen p53	polymer	40	4667.2	1	UniProt (P04637) Pfam (PF08563) Pfam (PF18521)	Homo sapiens (Human)	Antigen NY-CO-13,Phosphoprotein p53,Tumor suppressor p53
2	B (B)	S100P-ANXA2 chimera	polymer	512	57376.2	1	UniProt (P25815) UniProt (P07355) Pfam (PF01023) Pfam (PF00191)	Homo sapiens (Human)	Migration-inducing gene 9 protein,MIG9,Protein S100-E,S100 calcium-binding protein P,Protein S100-P,Annexin II,Annexin-2,Calpactin I heavy chain,Calpactin-1 heavy chain,Chromobindin-8,Lipocortin II,Placental anticoagulant protein IV,PAP-IV,Protein I,p36,Annexin A2
3	C (B)	GLYCEROL	non-polymer		92.1	1	Chemie (GOL)
4	D, E, F, G, H... (B)	CALCIUM ION	non-polymer		40.1	9	Chemie (CA)
5	M, N (A, B)	water	water		18.0	243	Chemie (HOH)

Sequence modifications

B: 1 - 95 (UniProt: P25815)

PDB	External Database	Details
His 0	-	expression tag

B: 104 - 198 (UniProt: P25815)

PDB	External Database	Details
Gly 96	-	linker
Gly 97	-	linker
Gly 98	-	linker
Gly 99	-	linker
Ser 100	-	linker
Gly 101	-	linker
Gly 102	-	linker
Ser 103	-	linker
Thr 199	-	linker
Ser 200	-	linker

B: 201 - 511 (UniProt: P07355)

PDB	External Database	Details
Glu 238	Ala 66	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	62043.4
Non-Polymers*	Number of molecules	10
	Total formula weight	452.8
All*	Total formula weight	62496.2

*Water molecules are not included.