7NMI

Transactivation domain of p53 in complex with S100P, using annexin A2 as crystallization chaperone

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-12-17
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1
Spacegroup name	P 21 21 21
Unit cell lengths	65.920, 86.770, 113.420
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.636 - 2.100
R-factor	0.1958
Rwork	0.194
R-free	0.22760
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1xjl
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.16_3549)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	47.636	47.636	2.150
High resolution limit [Å]	2.100	9.390	2.100
Rmerge	0.126	0.035	1.165
Rmeas	0.132	0.037	1.242
Total number of observations	450497
Number of reflections	38715	510	2821
<I/σ(I)>	22.47	69.26	3.02
Completeness [%]	99.9	98.6	99.8
Redundancy	11.636	11.661	9.359
CC(1/2)	0.998	0.999	0.528

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	Morpheus screen (MD) H6 0.1 M Amino acids, 0.1 M Buffer System 2, pH 7.5, 30 % v/v Precipitant Mix 2