7NJ0

CryoEM structure of the human Separase-Cdk1-cyclin B1-Cks1 complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Securin,Separin	polymer	2233	244677.3	1	UniProt (O95997) UniProt (Q14674) Pfam (PF03568)	Homo sapiens (Human)	Esp1-associated protein,Pituitary tumor-transforming gene 1 protein,Tumor-transforming protein 1,hPTTG,Caspase-like protein ESPL1,Extra spindle poles-like 1 protein,Separase
2	B (B)	Cyclin-dependent kinase 1	polymer	318	36667.1	1	UniProt (P06493) Pfam (PF00069)	Homo sapiens (Human)	CDK1,Cell division control protein 2 homolog,Cell division protein kinase 1,p34 protein kinase
3	C (C)	G2/mitotic-specific cyclin-B1,G2/mitotic-specific cyclin-B1	polymer	473	52625.7	1	UniProt (P14635) Pfam (PF00134) Pfam (PF02984)	Homo sapiens (Human)
4	D (D)	Cyclin-dependent kinases regulatory subunit 1	polymer	79	9679.2	1	UniProt (P61024) Pfam (PF01111)	Homo sapiens (Human)	CKS-1
5	E (D)	PHOSPHATE ION	non-polymer		95.0	1	Chemie (PO4)

Sequence modifications

A: -71 - -29 (UniProt: O95997)

PDB	External Database	Details
Met -72	-	initiating methionine
Gly -28	-	linker
Gly -27	-	linker
Ser -26	-	linker
Gly -25	-	linker
Gly -24	-	linker
Ser -23	-	linker
Gly -22	-	linker
Gly -21	-	linker
Gly -20	-	linker
Ser -19	-	linker
Gly -18	-	linker
Gly -17	-	linker
Gly -16	-	linker
Ser -15	-	linker
Gly -14	-	linker
Glu -13	-	linker
Asn -12	-	linker
Leu -11	-	linker
Tyr -10	-	linker
Phe -9	-	linker
Gln -8	-	linker
Gly -7	-	linker
Gly -6	-	linker
Gly -5	-	linker
Ser -4	-	linker
Gly -3	-	linker
Gly -2	-	linker
Ser -1	-	linker
Gly 0	-	linker

A: 1 - 2120 (UniProt: Q14674)

PDB	External Database	Details
Asp 25	Ala 25	conflict
Val 116	Ala 116	conflict
Ile 693	Met 693	conflict
Ser 1329	Arg 1329	conflict
Gln 1561	Arg 1561	conflict
Ser 2029	Cys 2029	conflict
His 2037	Arg 2037	conflict
Ser 2121	-	expression tag
Ser 2122	-	expression tag
Leu 2123	-	expression tag
Ala 2124	-	expression tag
Glu 2125	-	expression tag
Glu 2126	-	expression tag
Asn 2127	-	expression tag
Leu 2128	-	expression tag
Tyr 2129	-	expression tag
Phe 2130	-	expression tag
Gln 2131	-	expression tag
Ser 2132	-	expression tag
Trp 2133	-	expression tag
Ser 2134	-	expression tag
His 2135	-	expression tag
Pro 2136	-	expression tag
Gln 2137	-	expression tag
Phe 2138	-	expression tag
Glu 2139	-	expression tag
Lys 2140	-	expression tag
Gly 2141	-	expression tag
Gly 2142	-	expression tag
Gly 2143	-	expression tag
Ser 2144	-	expression tag
Gly 2145	-	expression tag
Gly 2146	-	expression tag
Gly 2147	-	expression tag
Ser 2148	-	expression tag
Gly 2149	-	expression tag
Gly 2150	-	expression tag
Gly 2151	-	expression tag
Ser 2152	-	expression tag
Trp 2153	-	expression tag
Ser 2154	-	expression tag
His 2155	-	expression tag
Pro 2156	-	expression tag
Gln 2157	-	expression tag
Phe 2158	-	expression tag
Glu 2159	-	expression tag
Lys 2160	-	expression tag

B: 1 - 297 (UniProt: P06493)

PDB	External Database	Details
Ile 298	-	expression tag
Ala 299	-	expression tag
Ala 300	-	expression tag
Glu 301	-	expression tag
Ala 302	-	expression tag
Leu 303	-	expression tag
Glu 304	-	expression tag
Val 305	-	expression tag
Leu 306	-	expression tag
Phe 307	-	expression tag
Gln 308	-	expression tag
Gly 309	-	expression tag
Pro 310	-	expression tag
His 311	-	expression tag
His 312	-	expression tag
His 313	-	expression tag
His 314	-	expression tag
His 315	-	expression tag
His 316	-	expression tag
His 317	-	expression tag
His 318	-	expression tag

C: 1 - 433 (UniProt: P14635)
C: 434 - 473 (PDB: 7NJ0)

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	343649.4
Non-Polymers*	Number of molecules	1
	Total formula weight	95.0
All*	Total formula weight	343744.3

*Water molecules are not included.