7NCX
Crystal structure of GH30 (double mutant EE) from Thermothelomyces thermophila.
This is a non-PDB format compatible entry.
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (AAA) | GH30 family xylanase | polymer | 482 | 51728.9 | 1 | UniProt (G2Q1N4) Pfam (PF14587) Pfam (PF17189) | Myceliophthora thermophila (strain ATCC 42464 / BCRC 31852 / DSM 1799) | |
| 2 | B (BBB) | alpha-D-mannopyranose-(1-3)-alpha-D-mannopyranose-(1-6)-[alpha-D-mannopyranose-(1-3)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 1073.0 | 1 | In PDB GlyTouCan (G09724ZC) | |||
| 3 | C (AAA) | GLYCEROL | non-polymer | 92.1 | 1 | Chemie (GOL) | |||
| 4 | D (AAA) | DI(HYDROXYETHYL)ETHER | non-polymer | 106.1 | 1 | Chemie (PEG) | |||
| 5 | E, F (AAA) | 1,2-ETHANEDIOL | non-polymer | 62.1 | 2 | Chemie (EDO) | |||
| 6 | G (AAA) | 2-acetamido-2-deoxy-beta-D-glucopyranose | non-polymer | 221.2 | 1 | Chemie (NAG) | |||
| 7 | H (AAA) | water | water | 18.0 | 515 | Chemie (HOH) |
Sequence modifications
AAA: 20 - 477 (UniProt: G2Q1N4)
| PDB | External Database | Details |
|---|---|---|
| Ser 18 | - | expression tag |
| Met 19 | - | expression tag |
| Ala 205 | Glu 205 | engineered mutation |
| Ala 295 | Glu 295 | engineered mutation |
| Leu 478 | - | expression tag |
| Glu 479 | - | expression tag |
| Gln 480 | - | expression tag |
| Lys 481 | - | expression tag |
| Leu 482 | - | expression tag |
| Ile 483 | - | expression tag |
| Ser 484 | - | expression tag |
| Glu 485 | - | expression tag |
| Glu 486 | - | expression tag |
| Asp 487 | - | expression tag |
| Leu 488 | - | expression tag |
| Asn 489 | - | expression tag |
| Ser 490 | - | expression tag |
| Ala 491 | - | expression tag |
| Val 492 | - | expression tag |
| Asp 493 | - | expression tag |
| His 494 | - | expression tag |
| His 495 | - | expression tag |
| His 496 | - | expression tag |
| His 497 | - | expression tag |
| His 498 | - | expression tag |
| His 499 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 1 |
| Total formula weight | 51728.9 | |
| Branched | Number of molecules | 1 |
| Total formula weight | 1073.0 | |
| Non-Polymers* | Number of molecules | 5 |
| Total formula weight | 543.6 | |
| All* | Total formula weight | 53345.5 |
