7NCX

Crystal structure of GH30 (double mutant EE) from Thermothelomyces thermophila.

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-02-07
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.9184
Spacegroup name	P 21 21 21
Unit cell lengths	38.887, 87.315, 107.917
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.696 - 1.360
Rwork	0.122
R-free	0.15810
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6krl
RMSD bond length	0.009
RMSD bond angle	1.576
Data reduction software	Aimless
Data scaling software	XDS
Phasing software	PHASER
Refinement software	REFMAC (5.8.0257)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.900	1.380
High resolution limit [Å]	1.360	1.360
Number of reflections	79578	3715
<I/σ(I)>	19.8
Completeness [%]	99.7
Redundancy	12.1
CC(1/2)	1.000	0.539

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	PEG1500, TBG buffer