7NBU
Structure of the HigB1 toxin mutant K95A from Mycobacterium tuberculosis (Rv1955) and its target, the cspA mRNA, on the E. coli Ribosome.
This is a non-PDB format compatible entry.
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | 16S ribosomal RNA | polymer | 1539 | 498846.8 | 1 | GenBank (817573384) | Escherichia coli | |
| 2 | B (B) | 30S ribosomal protein S2 | polymer | 224 | 24971.8 | 1 | UniProt (P0A7V0) Pfam (PF00318) | Escherichia coli K-12 | Small ribosomal subunit protein uS2 |
| 3 | C (C) | 30S ribosomal protein S3 | polymer | 206 | 23078.8 | 1 | UniProt (P0A7V3) Pfam (PF07650) Pfam (PF00189) | Escherichia coli K-12 | Small ribosomal subunit protein uS3 |
| 4 | D (D) | 30S ribosomal protein S4 | polymer | 205 | 23383.0 | 1 | UniProt (P0A7V8) Pfam (PF00163) Pfam (PF01479) | Escherichia coli K-12 | Small ribosomal subunit protein uS4 |
| 5 | E (E) | 30S ribosomal protein S5 | polymer | 156 | 16475.0 | 1 | UniProt (P0A7W1) Pfam (PF00333) Pfam (PF03719) | Escherichia coli K-12 | Small ribosomal subunit protein uS5 |
| 6 | F (F) | 30S ribosomal protein S6 | polymer | 103 | 11996.8 | 1 | UniProt (P02358) Pfam (PF01250) | Escherichia coli K-12 | Small ribosomal subunit protein bS6 |
| 7 | G (G) | 30S ribosomal protein S7 | polymer | 153 | 17162.8 | 1 | UniProt (A0A4S5AV58) | Escherichia coli K-12 | |
| 8 | H (H) | 30S ribosomal protein S8 | polymer | 129 | 14015.4 | 1 | UniProt (P0A7W7) Pfam (PF00410) | Escherichia coli K-12 | Small ribosomal subunit protein uS8 |
| 9 | I (I) | 30S ribosomal protein S9 | polymer | 127 | 14554.9 | 1 | UniProt (P0A7X3) Pfam (PF00380) | Escherichia coli K-12 | Small ribosomal subunit protein uS9 |
| 10 | J (J) | 30S ribosomal protein S10 | polymer | 98 | 11197.0 | 1 | UniProt (P0A7R5) Pfam (PF00338) | Escherichia coli K-12 | Small ribosomal subunit protein uS10 |
| 11 | K (K) | 30S ribosomal protein S11 | polymer | 117 | 12488.2 | 1 | UniProt (P0A7R9) Pfam (PF00411) | Escherichia coli K-12 | Small ribosomal subunit protein uS11 |
| 12 | L (L) | 30S ribosomal protein S12 | polymer | 123 | 13683.1 | 1 | UniProt (P0A7S3) Pfam (PF00164) | Escherichia coli K-12 | Small ribosomal subunit protein uS12 |
| 13 | M (M) | 30S ribosomal protein S13 | polymer | 115 | 12738.9 | 1 | UniProt (P0A7S9) Pfam (PF00416) | Escherichia coli K-12 | Small ribosomal subunit protein uS13 |
| 14 | N (N) | 30S ribosomal protein S14 | polymer | 100 | 11475.4 | 1 | UniProt (P0AG59) Pfam (PF00253) | Escherichia coli K-12 | Small ribosomal subunit protein uS14 |
| 15 | O (O) | 30S ribosomal protein S15 | polymer | 88 | 10159.6 | 1 | UniProt (P0ADZ4) Pfam (PF00312) | Escherichia coli K-12 | Small ribosomal subunit protein uS15 |
| 16 | P (P) | 30S ribosomal protein S16 | polymer | 81 | 9136.5 | 1 | UniProt (P0A7T3) Pfam (PF00886) | Escherichia coli K-12 | Small ribosomal subunit protein bS16 |
| 17 | Q (Q) | 30S ribosomal protein S17 | polymer | 79 | 9164.8 | 1 | UniProt (P0AG63) Pfam (PF00366) | Escherichia coli K-12 | Small ribosomal subunit protein uS17 |
| 18 | R (R) | 30S ribosomal protein S18 | polymer | 66 | 7735.9 | 1 | UniProt (P0A7T7) Pfam (PF01084) | Escherichia coli K-12 | Small ribosomal subunit protein bS18 |
| 19 | S (S) | 30S ribosomal protein S19 | polymer | 84 | 9492.1 | 1 | UniProt (P0A7U3) Pfam (PF00203) | Escherichia coli K-12 | Small ribosomal subunit protein uS19 |
| 20 | T (T) | 30S ribosomal protein S20 | polymer | 86 | 9577.3 | 1 | UniProt (P0A7U7) Pfam (PF01649) | Escherichia coli K-12 | Small ribosomal subunit protein bS20 |
| 21 | U (U) | 30S ribosomal protein S21 | polymer | 70 | 8392.8 | 1 | UniProt (P68679) Pfam (PF01165) | Escherichia coli K-12 | Small ribosomal subunit protein bS21 |
| 22 | V (V) | P-site fMet-tRNA(fMet) | polymer | 77 | 24818.9 | 1 | GenBank (731469900) | Escherichia coli K-12 | |
| 23 | W (W) | E-site tRNA | polymer | 2 | 589.4 | 1 | Escherichia coli K-12 | ||
| 24 | X (X) | cspA mRNA | polymer | 10 | 3144.9 | 1 | Mycobacterium tuberculosis H37Rv | ||
| 25 | Y (Y) | Probable endoribonuclease HigB1 | polymer | 121 | 14003.3 | 1 | UniProt (P9WJA5) Pfam (PF05973) | Mycobacterium tuberculosis H37Rv | Toxin HigB1 |
| 26 | Z (a) | 23S ribosomal RNA | polymer | 2904 | 941822.6 | 1 | Escherichia coli K-12 | ||
| 27 | AA (b) | 5S ribosomal RNA | polymer | 120 | 38790.1 | 1 | GenBank (1845258627) | Escherichia coli K-12 | |
| 28 | BA (c) | 50S ribosomal protein L2 | polymer | 271 | 29663.2 | 1 | UniProt (P60422) Pfam (PF00181) Pfam (PF03947) | Escherichia coli K-12 | Large ribosomal subunit protein uL2 |
| 29 | CA (d) | 50S ribosomal protein L3 | polymer | 209 | 22291.6 | 1 | UniProt (P60438) Pfam (PF00297) | Escherichia coli K-12 | Large ribosomal subunit protein uL3 |
| 30 | DA (e) | 50S ribosomal protein L4 | polymer | 201 | 22121.6 | 1 | UniProt (P60723) Pfam (PF00573) | Escherichia coli K-12 | Large ribosomal subunit protein uL4 |
| 31 | EA (f) | 50S ribosomal protein L5 | polymer | 177 | 20073.2 | 1 | UniProt (P62399) Pfam (PF00281) Pfam (PF00673) | Escherichia coli K-12 | Large ribosomal subunit protein uL5 |
| 32 | FA (g) | 50S ribosomal protein L6 | polymer | 176 | 18801.6 | 1 | UniProt (P0AG55) Pfam (PF00347) | Escherichia coli K-12 | Large ribosomal subunit protein uL6 |
| 33 | GA (h) | 50S ribosomal protein L9 | polymer | 41 | 4330.1 | 1 | UniProt (Q328J6) Pfam (PF01281) | Escherichia coli K-12 | |
| 34 | HA (i) | 50S ribosomal protein L13 | polymer | 142 | 16050.6 | 1 | UniProt (P0AA10) Pfam (PF00572) | Escherichia coli K-12 | Large ribosomal subunit protein uL13 |
| 35 | IA (j) | 50S ribosomal protein L14 | polymer | 123 | 13565.1 | 1 | UniProt (P0ADY3) Pfam (PF00238) | Escherichia coli K-12 | Large ribosomal subunit protein uL14 |
| 36 | JA (k) | 50S ribosomal protein L15 | polymer | 144 | 15008.5 | 1 | UniProt (P02413) Pfam (PF00828) | Escherichia coli K-12 | Large ribosomal subunit protein uL15 |
| 37 | KA (l) | 50S ribosomal protein L16,50S ribosomal protein L31 | polymer | 148 | 16900.0 | 1 | UniProt (P0ADY7) UniProt (P0A7M9) Pfam (PF00252) Pfam (PF01197) | Escherichia coli K-12 | Large ribosomal subunit protein uL16,Large ribosomal subunit protein bL31-A |
| 38 | LA (Z) | 50S ribosomal protein L16 | polymer | 54 | 5946.0 | 1 | UniProt (A0A3S4NPI3) Pfam (PF00252) | Escherichia coli K-12 | |
| 39 | MA (m) | 50S ribosomal protein L17 | polymer | 118 | 13505.7 | 1 | UniProt (P0AG44) Pfam (PF01196) | Escherichia coli K-12 | Large ribosomal subunit protein bL17 |
| 40 | NA (n) | 50S ribosomal protein L18 | polymer | 116 | 12663.5 | 1 | UniProt (P0C018) Pfam (PF00861) | Escherichia coli K-12 | Large ribosomal subunit protein uL18 |
| 41 | OA (o) | 50S ribosomal protein L19 | polymer | 114 | 13028.1 | 1 | UniProt (P0A7K6) Pfam (PF01245) | Escherichia coli K-12 | Large ribosomal subunit protein bL19 |
| 42 | PA (p) | 50S ribosomal protein L20 | polymer | 117 | 13396.8 | 1 | UniProt (P0A7L3) Pfam (PF00453) | Escherichia coli K-12 | Large ribosomal subunit protein bL20 |
| 43 | QA (q) | 50S ribosomal protein L21 | polymer | 103 | 11586.4 | 1 | UniProt (P0AG48) Pfam (PF00829) | Escherichia coli K-12 | Large ribosomal subunit protein bL21 |
| 44 | RA (r) | 50S ribosomal protein L22 | polymer | 110 | 12253.4 | 1 | UniProt (P61175) Pfam (PF00237) | Escherichia coli K-12 | Large ribosomal subunit protein uL22 |
| 45 | SA (s) | 50S ribosomal protein L23 | polymer | 93 | 10546.5 | 1 | UniProt (P0ADZ0) Pfam (PF00276) | Escherichia coli K-12 | Large ribosomal subunit protein uL23 |
| 46 | TA (t) | 50S ribosomal protein L24 | polymer | 102 | 11078.9 | 1 | UniProt (P60624) Pfam (PF00467) Pfam (PF17136) | Escherichia coli K-12 | Large ribosomal subunit protein uL24 |
| 47 | UA (u) | 50S ribosomal protein L25 | polymer | 94 | 10713.5 | 1 | UniProt (P68919) Pfam (PF01386) | Escherichia coli K-12 | Large ribosomal subunit protein bL25 |
| 48 | VA (v) | 50S ribosomal protein L27 | polymer | 84 | 9015.3 | 1 | UniProt (P0A7L8) Pfam (PF01016) | Escherichia coli K-12 | Large ribosomal subunit protein bL27 |
| 49 | WA (w) | 50S ribosomal protein L28 | polymer | 77 | 8896.4 | 1 | UniProt (P0A7M2) Pfam (PF00830) | Escherichia coli K-12 | Large ribosomal subunit protein bL28 |
| 50 | XA (x) | 50S ribosomal protein L29 | polymer | 62 | 7155.3 | 1 | UniProt (P0A7M6) Pfam (PF00831) | Escherichia coli K-12 | Large ribosomal subunit protein uL29 |
| 51 | YA (y) | 50S ribosomal protein L30 | polymer | 58 | 6423.6 | 1 | UniProt (P0AG51) Pfam (PF00327) | Escherichia coli K-12 | Large ribosomal subunit protein uL30 |
| 52 | ZA (z) | 50S ribosomal protein L32 | polymer | 56 | 6332.2 | 1 | UniProt (P0A7N4) Pfam (PF01783) | Escherichia coli K-12 | Large ribosomal subunit protein bL32 |
| 53 | AB (0) | 50S ribosomal protein L33 | polymer | 51 | 5928.0 | 1 | UniProt (P0A7N9) Pfam (PF00471) | Escherichia coli K-12 | Large ribosomal subunit protein bL33 |
| 54 | BB (1) | 50S ribosomal protein L34 | polymer | 46 | 5397.5 | 1 | UniProt (P0A7P5) Pfam (PF00468) | Escherichia coli K-12 | Large ribosomal subunit protein bL34 |
| 55 | CB (2) | 50S ribosomal protein L35 | polymer | 64 | 7181.8 | 1 | UniProt (P0A7Q1) Pfam (PF01632) | Escherichia coli K-12 | Large ribosomal subunit protein bL35,Ribosomal protein A |
| 56 | DB (3) | 50S ribosomal protein L36 | polymer | 38 | 4377.4 | 1 | UniProt (P0A7Q6) Pfam (PF00444) | Escherichia coli K-12 | Large ribosomal subunit protein bL36-A,Ribosomal protein B |
| 57 | AC, AD, AE, AF, AG... (A, a, c, d, m...) | MAGNESIUM ION | non-polymer | 24.3 | 313 | Chemie (MG) | |||
| 58 | XD (V) | N-FORMYLMETHIONINE | non-polymer | 177.2 | 1 | Chemie (FME) | |||
| 59 | EN, HN (l, 3) | ZINC ION | non-polymer | 65.4 | 2 | Chemie (ZN) |
Sequence modifications
K: 13 - 129 (UniProt: P0A7R9)
Y: 7 - 125 (UniProt: P9WJA5)
l: 1 - 80 (UniProt: P0ADY7)
l: 83 - 148 (UniProt: P0A7M9)
| PDB | External Database | Details |
|---|---|---|
| Ias 119 | Asn 119 | conflict |
| PDB | External Database | Details |
|---|---|---|
| Ala 95 | Lys 95 | engineered mutation |
| Leu 126 | - | expression tag |
| Glu 127 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| 4d4 81 | - | linker |
| Ms6 82 | - | linker |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 56 |
| Total formula weight | 2147127.7 | |
| Non-Polymers* | Number of molecules | 316 |
| Total formula weight | 7915.5 | |
| All* | Total formula weight | 2155043.2 |
