7N3Y
Crystal Structure of Saccharomyces cerevisiae Apn2 Catalytic Domain E59Q/D222N Mutant in Complex with DNA
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B, C, D (A, B, C, D) | DNA-(apurinic or apyrimidinic site) endonuclease 2 | polymer | 413 | 47322.1 | 4 | UniProt (P38207) Pfam (PF03372) | Saccharomyces cerevisiae (Baker's yeast) | AP endonuclease 2,Apurinic-apyrimidinic endonuclease 2 |
| 2 | E, F, G (E, F, G) | DNA (5'-D(*TP*CP*CP*GP*AP*AP*AP*TP*(PST)P*(PST)P*(SC)P*(GS)P*(GS))-3') | polymer | 13 | 4046.9 | 3 | synthetic construct | ||
| 3 | H (H) | DNA (5'-D(*CP*CP*GP*AP*AP*AP*TP*TP*(PST)P*(SC)P*(GS)P*(GS)P*(AS))-3') | polymer | 13 | 4055.9 | 1 | synthetic construct | ||
| 4 | I, N (A, C) | D(-)-TARTARIC ACID | non-polymer | 150.1 | 2 | Chemie (TAR) | |||
| 5 | J (A) | CALCIUM ION | non-polymer | 40.1 | 1 | Chemie (CA) | |||
| 6 | K, O (B, D) | L(+)-TARTARIC ACID | non-polymer | 150.1 | 2 | Chemie (TLA) | |||
| 7 | L (B) | CITRIC ACID | non-polymer | 192.1 | 1 | Chemie (CIT) | |||
| 8 | M (B) | 1,2-ETHANEDIOL | non-polymer | 62.1 | 1 | Chemie (EDO) | |||
| 9 | P, Q, R, S (A, B, E, F) | water | water | 18.0 | 100 | Chemie (HOH) |
Sequence modifications
A, B, C, D: 1 - 407 (UniProt: P38207)
| PDB | External Database | Details |
|---|---|---|
| Gln 59 | Glu 59 | engineered mutation |
| Asn 222 | Asp 222 | engineered mutation |
| Glu 408 | - | expression tag |
| Asn 409 | - | expression tag |
| Leu 410 | - | expression tag |
| Tyr 411 | - | expression tag |
| Phe 412 | - | expression tag |
| Gln 413 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 8 |
| Total formula weight | 205485.1 | |
| Non-Polymers* | Number of molecules | 7 |
| Total formula weight | 894.6 | |
| All* | Total formula weight | 206379.7 |
