7N3Y

Crystal Structure of Saccharomyces cerevisiae Apn2 Catalytic Domain E59Q/D222N Mutant in Complex with DNA

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D	DNA-(apurinic or apyrimidinic site) endonuclease 2	polymer	413	47322.1	4	UniProt (P38207) Pfam (PF03372) In PDB	Saccharomyces cerevisiae (Baker's yeast)	AP endonuclease 2,Apurinic-apyrimidinic endonuclease 2
2	E, F, G	DNA (5'-D(*TP*CP*CP*GP*AP*AP*AP*TP*(PST)P*(PST)P*(SC)P*(GS)P*(GS))-3')	polymer	13	4046.9	3		synthetic construct
3	H	DNA (5'-D(*CP*CP*GP*AP*AP*AP*TP*TP*(PST)P*(SC)P*(GS)P*(GS)P*(AS))-3')	polymer	13	4055.9	1		synthetic construct
4	A, C	D(-)-TARTARIC ACID	non-polymer		150.1	2	Chemie (TAR)
5	A	CALCIUM ION	non-polymer		40.1	1	Chemie (CA)
6	B, D	L(+)-TARTARIC ACID	non-polymer		150.1	2	Chemie (TLA)
7	B	CITRIC ACID	non-polymer		192.1	1	Chemie (CIT)
8	B	1,2-ETHANEDIOL	non-polymer		62.1	1	Chemie (EDO)
9		water	water		18.0	100	Chemie (HOH)

Sequence modifications

A, B, C, D: 1 - 407 (UniProt: P38207)

PDB	External Database	Details
Gln 59	Glu 59	engineered mutation
Asn 222	Asp 222	engineered mutation
Glu 408	-	expression tag
Asn 409	-	expression tag
Leu 410	-	expression tag
Tyr 411	-	expression tag
Phe 412	-	expression tag
Gln 413	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	8
	Total formula weight	205485.1
Non-Polymers*	Number of molecules	7
	Total formula weight	894.6
All*	Total formula weight	206379.7

*Water molecules are not included.