7N0D
Cryo-EM structure of the tetrameric form of SARS-CoV-2 nsp10-nsp14 (E191A)-RNA complex
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, C, H, J (A, C, E, G) | Non-structural protein 10 | polymer | 139 | 14802.9 | 4 | UniProt (P0DTD1) Pfam (PF09401) | Severe acute respiratory syndrome coronavirus 2 (2019-nCoV SARS-CoV-2) | nsp10,Growth factor-like peptide,GFL |
| 2 | B, D, I, K (B, D, F, H) | Proofreading exoribonuclease | polymer | 527 | 59829.4 | 4 | UniProt (P0DTD1) Pfam (PF06471) | Severe acute respiratory syndrome coronavirus 2 (2019-nCoV SARS-CoV-2) | ExoN,Guanine-N7 methyltransferase,Non-structural protein 14,nsp14 |
| 3 | E, L (T, I) | RNA (5'-R(*GP*GP*GP*GP*AP*UP*GP*UP*GP*AP*UP*UP*UP*UP*AP*AP*UP*AP*G)-3') | polymer | 27 | 8608.0 | 2 | Severe acute respiratory syndrome coronavirus 2 | ||
| 4 | F, M (K, L) | RNA (5'-R(*CP*UP*AP*UP*UP*AP*AP*AP*AP*UP*CP*AP*CP*C)-3') | polymer | 22 | 7033.3 | 2 | Severe acute respiratory syndrome coronavirus 2 | ||
| 5 | G, N (P, J) | RNA (5'-R(*CP*CP*CP*CP*C)-3') | polymer | 5 | 1481.0 | 2 | Severe acute respiratory syndrome coronavirus 2 | ||
| 6 | AA, CA, DA, GA, HA... (D, E, F, G, H...) | ZINC ION | non-polymer | 65.4 | 20 | Chemie (ZN) | |||
| 7 | EA, FA, MA, Q, R... (F, H, B, D) | MAGNESIUM ION | non-polymer | 24.3 | 6 | Chemie (MG) PubChem (888) | |||
| 8 | BA, LA (D, H) | CHAPSO | non-polymer | 631.9 | 2 | Chemie (1N7) PubChem (122146) PubChem (3253739) PubChem (9895487) PubChem (16402373) PubChem (44119969) PubChem (44660322) PubChem (23636791) PubChem (23639399) PubChem (50987332) PubChem (71305076) PubChem (90479263) PubChem (96545191) PubChem (53393747) PubChem (124745618) PubChem (124901558) PubChem (124905421) PubChem (125027410) PubChem (10009199) PubChem (144605089) PubChem (71464051) PubChem (96327541) PubChem (96327542) PubChem (96327543) PubChem (96327544) PubChem (96545192) PubChem (102565431) PubChem (121392376) PubChem (122130148) PubChem (124901556) PubChem (124901561) PubChem (124901563) PubChem (124905422) PubChem (125027411) PubChem (129537355) PubChem (129537356) PubChem (145705472) PubChem (146030291) PubChem (146159852) | |||
| 9 | QA, RA, SA, TA (B, D, F, H) | water | water | 18.0 | 4 | Chemie (HOH) |
Sequence modifications
B, D, F, H: 1 - 527 (UniProt: P0DTD1)
| PDB | External Database | Details |
|---|---|---|
| Ala 191 | Glu 6116 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 14 |
| Total formula weight | 332774.0 | |
| Non-Polymers* | Number of molecules | 28 |
| Total formula weight | 2717.8 | |
| All* | Total formula weight | 335491.8 |
