7N0D
Cryo-EM structure of the tetrameric form of SARS-CoV-2 nsp10-nsp14 (E191A)-RNA complex
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A, C, E, G | Non-structural protein 10 | polymer | 139 | 14802.9 | 4 | UniProt (P0DTD1) Pfam (PF09401) In PDB | Severe acute respiratory syndrome coronavirus 2 (2019-nCoV SARS-CoV-2) | nsp10,Growth factor-like peptide,GFL |
2 | B, D, F, H | Proofreading exoribonuclease | polymer | 527 | 59829.4 | 4 | UniProt (P0DTD1) Pfam (PF06471) In PDB | Severe acute respiratory syndrome coronavirus 2 (2019-nCoV SARS-CoV-2) | ExoN,Guanine-N7 methyltransferase,Non-structural protein 14,nsp14 |
3 | T, I | RNA (5'-R(*GP*GP*GP*GP*AP*UP*GP*UP*GP*AP*UP*UP*UP*UP*AP*AP*UP*AP*G)-3') | polymer | 27 | 8608.0 | 2 | Severe acute respiratory syndrome coronavirus 2 | ||
4 | K, L | RNA (5'-R(*CP*UP*AP*UP*UP*AP*AP*AP*AP*UP*CP*AP*CP*C)-3') | polymer | 22 | 7033.3 | 2 | Severe acute respiratory syndrome coronavirus 2 | ||
5 | P, J | RNA (5'-R(*CP*CP*CP*CP*C)-3') | polymer | 5 | 1481.0 | 2 | Severe acute respiratory syndrome coronavirus 2 | ||
6 | D, E, F, G, H... | ZINC ION | non-polymer | 65.4 | 20 | Chemie (ZN) | |||
7 | F, H, B, D | MAGNESIUM ION | non-polymer | 24.3 | 6 | Chemie (MG) | |||
8 | D, H | CHAPSO | non-polymer | 631.9 | 2 | Chemie (1N7) | |||
9 | water | water | 18.0 | 4 | Chemie (HOH) |
Sequence modifications
B, D, F, H: 1 - 527 (UniProt: P0DTD1)
PDB | External Database | Details |
---|---|---|
Ala 191 | Glu 6116 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 14 |
Total formula weight | 332774.0 | |
Non-Polymers* | Number of molecules | 28 |
Total formula weight | 2717.8 | |
All* | Total formula weight | 335491.8 |