7N0D

Cryo-EM structure of the tetrameric form of SARS-CoV-2 nsp10-nsp14 (E191A)-RNA complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C, H, J (A, C, E, G)	Non-structural protein 10	polymer	139	14802.9	4	UniProt (P0DTD1) Pfam (PF09401)	Severe acute respiratory syndrome coronavirus 2 (2019-nCoV SARS-CoV-2)	nsp10,Growth factor-like peptide,GFL
2	B, D, I, K (B, D, F, H)	Proofreading exoribonuclease	polymer	527	59829.4	4	UniProt (P0DTD1) Pfam (PF06471)	Severe acute respiratory syndrome coronavirus 2 (2019-nCoV SARS-CoV-2)	ExoN,Guanine-N7 methyltransferase,Non-structural protein 14,nsp14
3	E, L (T, I)	RNA (5'-R(*GP*GP*GP*GP*AP*UP*GP*UP*GP*AP*UP*UP*UP*UP*AP*AP*UP*AP*G)-3')	polymer	27	8608.0	2		Severe acute respiratory syndrome coronavirus 2
4	F, M (K, L)	RNA (5'-R(*CP*UP*AP*UP*UP*AP*AP*AP*AP*UP*CP*AP*CP*C)-3')	polymer	22	7033.3	2		Severe acute respiratory syndrome coronavirus 2
5	G, N (P, J)	RNA (5'-R(*CP*CP*CP*CP*C)-3')	polymer	5	1481.0	2		Severe acute respiratory syndrome coronavirus 2
6	AA, CA, DA, GA, HA... (D, E, F, G, H...)	ZINC ION	non-polymer		65.4	20	Chemie (ZN)
7	EA, FA, MA, Q, R... (F, H, B, D)	MAGNESIUM ION	non-polymer		24.3	6	Chemie (MG)
8	BA, LA (D, H)	CHAPSO	non-polymer		631.9	2	Chemie (1N7)
9	QA, RA, SA, TA (B, D, F, H)	water	water		18.0	4	Chemie (HOH)

Sequence modifications

B, D, F, H: 1 - 527 (UniProt: P0DTD1)

PDB	External Database	Details
Ala 191	Glu 6116	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	14
	Total formula weight	332774.0
Non-Polymers*	Number of molecules	28
	Total formula weight	2717.8
All*	Total formula weight	335491.8

*Water molecules are not included.