7MYF

Ubiquitin variant UbV.k.1 in complex with Ube2k

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Ubiquitin-conjugating enzyme E2 K	polymer	201	22555.7	1	UniProt (P61086) Pfam (PF00179) Pfam (PF00627)	Homo sapiens (Human)	E2 ubiquitin-conjugating enzyme K,Huntingtin-interacting protein 2,HIP-2,Ubiquitin carrier protein,Ubiquitin-conjugating enzyme E2-25 kDa,Ubiquitin-conjugating enzyme E2(25K),Ubiquitin-conjugating enzyme E2-25K,Ubiquitin-protein ligase
2	B (B)	Ubiquitin variant UbV.k.1	polymer	94	10681.9	1	UniProt (P0CG47) Pfam (PF00240)	Homo sapiens (Human)
3	C (C)	Ubiquitin	polymer	76	8576.8	1	UniProt (P0CG48) Pfam (PF00240)	Homo sapiens (Human)

Sequence modifications

A: 2 - 200 (UniProt: P61086)

PDB	External Database	Details
Met 0	-	expression tag
Ala 1	-	expression tag
Lys 92	Cys 92	engineered mutation
Arg 97	Lys 97	engineered mutation

B: 1 - 75 (UniProt: P0CG47)

PDB	External Database	Details
Gly -10	-	expression tag
Pro -9	-	expression tag
Gly -8	-	expression tag
Asp -7	-	expression tag
Tyr -6	-	expression tag
Lys -5	-	expression tag
Asp -4	-	expression tag
Asp -3	-	expression tag
Asp -2	-	expression tag
Asp -1	-	expression tag
Lys 0	-	expression tag
Phe 2	Gln 2	engineered mutation
Arg 10	Gly 10	engineered mutation
Tyr 12	Thr 12	engineered mutation
Ser 46	Ala 46	engineered mutation
Gln 48	Lys 48	engineered mutation
Glu 63	Lys 63	engineered mutation
Phe 64	Glu 64	engineered mutation
Phe 66	Thr 66	engineered mutation
Ile 70	Val 70	engineered mutation
Ser 71	Leu 71	engineered mutation
Val 76	-	expression tag
Tyr 77	-	expression tag
Gln 78	-	expression tag
Gly 79	-	expression tag
Gly 80	-	expression tag
Ser 81	-	expression tag
Gly 82	-	expression tag
Gly 83	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	41814.5
All*	Total formula weight	41814.5

*Water molecules are not included.