7MYF
Ubiquitin variant UbV.k.1 in complex with Ube2k
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
Synchrotron site | Australian Synchrotron |
Beamline | MX2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-09-26 |
Detector | DECTRIS EIGER2 X 16M |
Wavelength(s) | 0.9537 |
Spacegroup name | I 1 2 1 |
Unit cell lengths | 89.144, 37.792, 117.797 |
Unit cell angles | 90.00, 98.27, 90.00 |
Refinement procedure
Resolution | 37.892 - 3.000 |
R-factor | 0.237 |
Rwork | 0.234 |
R-free | 0.29330 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5dfl |
Data reduction software | XDS |
Data scaling software | Aimless (0.7.4) |
Phasing software | PHASER |
Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 44.110 | 44.110 | 3.180 |
High resolution limit [Å] | 2.990 | 8.980 | 2.990 |
Rmerge | 0.170 | 0.088 | 0.829 |
Rmeas | 0.191 | 0.100 | 0.933 |
Rpim | 0.085 | 0.046 | 0.419 |
Total number of observations | 36340 | 1415 | 5956 |
Number of reflections | 7820 | 329 | 1285 |
<I/σ(I)> | 6.2 | 14.2 | 1.9 |
Completeness [%] | 96.3 | 98.9 | 98.3 |
Redundancy | 4.6 | 4.3 | 4.6 |
CC(1/2) | 0.991 | 0.993 | 0.683 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 289 | 0.2-0.3 M ammonium citrate dibasic, 20-25% PEG3350 |