7MXN

PRMT5(M420T mutant):MEP50 complexed with inhibitor PF-06939999

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Protein arginine N-methyltransferase 5	polymer	637	72736.6	1	UniProt (O14744) Pfam (PF17285) Pfam (PF05185) Pfam (PF17286) In PDB	Homo sapiens (Human)	PRMT5,72 kDa ICln-binding protein,Histone-arginine N-methyltransferase PRMT5,Jak-binding protein 1,Shk1 kinase-binding protein 1 homolog,SKB1 homolog,SKB1Hs
2	B	Methylosome protein 50	polymer	366	39520.1	1	UniProt (Q9BQA1) Pfam (PF00400) In PDB	Homo sapiens (Human)	MEP-50,Androgen receptor cofactor p44,WD repeat-containing protein 77,p44/Mep50
3	A	(1S,2S,3S,5R)-3-{[6-(difluoromethyl)-5-fluoro-1,2,3,4-tetrahydroisoquinolin-8-yl]oxy}-5-(4-methyl-7H-pyrrolo[2,3-d]pyrimidin-7-yl)cyclopentane-1,2-diol	non-polymer		448.4	1	Chemie (ZR1)
4		water	water		18.0	237	Chemie (HOH)

Sequence modifications

A: 1 - 637 (UniProt: O14744)

PDB	External Database	Details
Thr 420	Met 420	engineered mutation

B: 2 - 342 (UniProt: Q9BQA1)

PDB	External Database	Details
Met -23	-	expression tag
Ala -22	-	expression tag
Ser -21	-	expression tag
His -20	-	expression tag
His -19	-	expression tag
His -18	-	expression tag
His -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
Asp -14	-	expression tag
Tyr -13	-	expression tag
Asp -12	-	expression tag
Gly -11	-	expression tag
Ala -10	-	expression tag
Thr -9	-	expression tag
Thr -8	-	expression tag
Glu -7	-	expression tag
Asn -6	-	expression tag
Leu -5	-	expression tag
Tyr -4	-	expression tag
Phe -3	-	expression tag
Gln -2	-	expression tag
Gly -1	-	expression tag
Ser 0	-	expression tag
Leu 1	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	112256.7
Non-Polymers*	Number of molecules	1
	Total formula weight	448.4
All*	Total formula weight	112705.1

*Water molecules are not included.