7MOP

Cryo-EM structure of human HUWE1 in complex with DDIT4

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	E3 ubiquitin-protein ligase HUWE1	polymer	4411	486409.5	1	UniProt (Q7Z6Z7) Pfam (PF06012) Pfam (PF06025) Pfam (PF22562) Pfam (PF02825) Pfam (PF14377) Pfam (PF00632) In PDB	Homo sapiens (Human)	ARF-binding protein 1,ARF-BP1,HECT,UBA and WWE domain-containing protein 1,HECT-type E3 ubiquitin transferase HUWE1,Homologous to E6AP carboxyl terminus homologous protein 9,HectH9,Large structure of UREB1,LASU1,Mcl-1 ubiquitin ligase E3,Mule,Upstream regulatory element-binding protein 1,URE-binding protein 1
2	B	DNA damage-inducible transcript 4 protein	polymer	259	28436.8	1	UniProt (Q9NX09) Pfam (PF07809) In PDB	Homo sapiens (Human)	HIF-1 responsive protein RTP801,Protein regulated in development and DNA damage response 1,REDD-1

Sequence modifications

A: 1 - 4374 (UniProt: Q7Z6Z7)

PDB	External Database	Details
Met -36	-	expression tag
Asp -35	-	expression tag
Tyr -34	-	expression tag
Lys -33	-	expression tag
Asp -32	-	expression tag
Asp -31	-	expression tag
Asp -30	-	expression tag
Asp -29	-	expression tag
Lys -28	-	expression tag
Leu -27	-	expression tag
Ala -26	-	expression tag
Ala -25	-	expression tag
Ala -24	-	expression tag
Asn -23	-	expression tag
Ser -22	-	expression tag
Ser -21	-	expression tag
Ile -20	-	expression tag
Asp -19	-	expression tag
Leu -18	-	expression tag
Ile -17	-	expression tag
Ser -16	-	expression tag
Thr -15	-	expression tag
Ser -14	-	expression tag
Leu -13	-	expression tag
Tyr -12	-	expression tag
Lys -11	-	expression tag
Lys -10	-	expression tag
Ala -9	-	expression tag
Gly -8	-	expression tag
Phe -7	-	expression tag
Lys -6	-	expression tag
Gly -5	-	expression tag
Thr -4	-	expression tag
Asn -3	-	expression tag
Ser -2	-	expression tag
Val -1	-	expression tag
Asp 0	-	expression tag

B: 1 - 232 (UniProt: Q9NX09)

PDB	External Database	Details
Met -26	-	expression tag
Asp -25	-	expression tag
Ser -24	-	expression tag
Ala -23	-	expression tag
Trp -22	-	expression tag
Ser -21	-	expression tag
His -20	-	expression tag
Pro -19	-	expression tag
Gln -18	-	expression tag
Phe -17	-	expression tag
Glu -16	-	expression tag
Lys -15	-	expression tag
Ser -14	-	expression tag
Ala -13	-	expression tag
Val -12	-	expression tag
Asp -11	-	expression tag
Glu -10	-	expression tag
Asn -9	-	expression tag
Leu -8	-	expression tag
Tyr -7	-	expression tag
Phe -6	-	expression tag
Gln -5	-	expression tag
Gly -4	-	expression tag
Gly -3	-	expression tag
Gly -2	-	expression tag
Arg -1	-	expression tag
Thr 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	514846.4
All*	Total formula weight	514846.4

*Water molecules are not included.