7MNU

Crystal Structure of the ZnF7 of Nucleoporin NUP358/RanBP2 in complex with Ran-GDP

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	GTP-binding nuclear protein Ran	polymer	236	26567.4	1	UniProt (P62826) Pfam (PF00071)	Homo sapiens (Human)	Androgen receptor-associated protein 24,GTPase Ran,Ras-like protein TC4,Ras-related nuclear protein
2	B (B)	E3 SUMO-protein ligase RanBP2	polymer	43	4604.4	1	UniProt (P49792) Pfam (PF00641)	Homo sapiens (Human)	358 kDa nucleoporin,Nuclear pore complex protein Nup358,Nucleoporin Nup358,Ran-binding protein 2,RanBP2,p270
3	C (A)	GUANOSINE-5'-DIPHOSPHATE	non-polymer		443.2	1	Chemie (GDP)
4	D (A)	MAGNESIUM ION	non-polymer		24.3	1	Chemie (MG)
5	E (B)	ZINC ION	non-polymer		65.4	1	Chemie (ZN)
6	F, G (A, B)	water	water		18.0	196	Chemie (HOH)

Sequence modifications

A: 1 - 216 (UniProt: P62826)

PDB	External Database	Details
Met -19	-	expression tag
Gly -18	-	expression tag
Ser -17	-	expression tag
Ser -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
Ser -9	-	expression tag
Ser -8	-	expression tag
Gly -7	-	expression tag
Leu -6	-	expression tag
Val -5	-	expression tag
Pro -4	-	expression tag
Arg -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag
Ser 35	Phe 35	engineered mutation

B: 1716 - 1752 (UniProt: P49792)

PDB	External Database	Details
Gly 1710	-	expression tag
Pro 1711	-	expression tag
Leu 1712	-	expression tag
Gly 1713	-	expression tag
Ser 1714	-	expression tag
Met 1715	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	31171.7
Non-Polymers*	Number of molecules	3
	Total formula weight	532.9
All*	Total formula weight	31704.7

*Water molecules are not included.