7MJS

Single-Particle Cryo-EM Structure of Major Facilitator Superfamily Domain containing 2A in complex with LPC-18:3

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	H	2AG3 Fab heavy chain	polymer	240	25646.5	1		Synthetic construct
2	L	2AG3 Fab light chain	polymer	217	23655.2	1		Synthetic construct
3	X	Major Facilitator Superfamily Domain containing 2A	polymer	546	60789.2	1	UniProt (F1NCD6) Pfam (PF13347) In PDB	Gallus gallus (Chicken)
4	X	[(2~{R})-2-oxidanyl-3-[oxidanyl-[2-(trimethyl-$l^{4}-azanyl)ethoxy]phosphoryl]oxy-propyl] (9~{Z},12~{Z},15~{Z})-octadeca-9,12,15-trienoate	non-polymer		518.6	1	Chemie (ZGS)
5	X	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	2	Chemie (NAG)

Sequence modifications

X: 1 - 528 (UniProt: F1NCD6)

PDB	External Database	Details
Glu 529	-	expression tag
Asn 530	-	expression tag
Leu 531	-	expression tag
Tyr 532	-	expression tag
Phe 533	-	expression tag
Gln 534	-	expression tag
Gly 535	-	expression tag
Ser 536	-	expression tag
His 537	-	expression tag
His 538	-	expression tag
His 539	-	expression tag
His 540	-	expression tag
His 541	-	expression tag
His 542	-	expression tag
His 543	-	expression tag
His 544	-	expression tag
His 545	-	expression tag
His 546	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	110090.9
Non-Polymers*	Number of molecules	3
	Total formula weight	961.1
All*	Total formula weight	111051.9

*Water molecules are not included.