7MHW

Crystal structure of the protease inhibitor U-Omp19 from Brucella abortus fused to Maltose-binding protein

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Maltose/maltodextrin-binding periplasmic protein,Outer membrane lipoprotein omp19	polymer	481	51734.2	1	UniProt (P0AEX9) UniProt (Q2YLR6) Pfam (PF01547) Pfam (PF02974)	Escherichia coli
2	B, C, D, E, F... (A)	SULFATE ION	non-polymer		96.1	7	Chemie (SO4)
3	I (A)	water	water		18.0	37	Chemie (HOH)

Sequence modifications

A: -314 - 51 (UniProt: P0AEX9)

PDB	External Database	Details
Met -315	-	initiating methionine
Ala -233	Asp 108	engineered mutation
Ala -232	Lys 109	engineered mutation
Ala -76	Lys 265	engineered mutation
Ala 44	Glu 385	engineered mutation
Ala 47	Lys 388	engineered mutation
Ala 48	Asp 389	engineered mutation
Asn 52	-	linker
Ala 53	-	linker
Ala 54	-	linker
Ala 55	-	linker
Met 56	-	linker

A: 57 - 157 (UniProt: Q2YLR6)

PDB	External Database	Details
Leu 158	-	expression tag
Glu 159	-	expression tag
His 160	-	expression tag
His 161	-	expression tag
His 162	-	expression tag
His 163	-	expression tag
His 164	-	expression tag
His 165	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	51734.2
Non-Polymers*	Number of molecules	7
	Total formula weight	672.4
All*	Total formula weight	52406.7

*Water molecules are not included.