7MEM

CryoEM structure of monoclonal Fab 045-09 2B05 binding the lateral patch of influenza virus H1 HA

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, E, I (E, C, F)	Hemagglutinin HA1 chain	polymer	331	36729.4	3	UniProt (C3W5S1) Pfam (PF00509)	Influenza A virus (strain swl A/California/04/2009 H1N1)
2	B, F, J (L, J, K)	Light chain of monoclonal antibody 045-09 2B05	polymer	107	11769.1	3		Homo sapiens
3	C, G, K (H, G, I)	Heavy chain of monoclonal antibody 045-09 2B05	polymer	120	12805.2	3		Homo sapiens
4	D, H, L (D, A, B)	Hemagglutinin HA2 chain	polymer	174	19905.0	3	UniProt (C3W5S1) Pfam (PF00509)	Influenza A virus (strain swl A/California/04/2009 H1N1)
5	M, O, Q (M, O, Q)	alpha-L-fucopyranose-(1-6)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		367.3	3	In PDB GlyTouCan (G86851RC)
6	N, P, R (N, P, R)	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		424.4	3	In PDB GlyTouCan (G42666HT)
7	S, T, U, V, W... (E, C, F)	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	6	Chemie (NAG)

Sequence modifications

E, C, F: 11 - 329 (UniProt: C3W5S1)

PDB	External Database	Details
Ala 7	-	expression tag
Asp 8	-	expression tag
Pro 9	-	expression tag
Gly 10	-	expression tag

D, A, B: 1 - 174 (UniProt: C3W5S1)

PDB	External Database	Details
Gly 47	Glu 391	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	12
	Total formula weight	243626.3
Branched	Number of molecules	6
	Total formula weight	2375.3
Non-Polymers*	Number of molecules	6
	Total formula weight	1327.2
All*	Total formula weight	247328.8

*Water molecules are not included.