7M45
DNA Polymerase Lambda, TTP:At Mg2+ Reaction State Ternary Complex, 120 sec
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | DNA polymerase lambda | polymer | 329 | 36704.0 | 1 | UniProt (Q9UGP5) Pfam (PF14716) Pfam (PF10391) Pfam (PF14792) Pfam (PF14791) | Homo sapiens (human) | Pol Lambda,DNA polymerase beta-2,Pol beta2,DNA polymerase kappa |
| 2 | B (T) | DNA (5'-D(*CP*GP*GP*CP*AP*GP*TP*AP*CP*TP*G)-3') | polymer | 11 | 3374.2 | 1 | synthetic construct | ||
| 3 | C (P) | DNA (5'-D(*CP*AP*GP*TP*AP*CP*T)-3') | polymer | 7 | 2097.4 | 1 | synthetic construct | ||
| 4 | D (D) | DNA (5'-D(P*GP*CP*CP*G)-3') | polymer | 4 | 1191.8 | 1 | synthetic construct | ||
| 5 | E (A) | PYROPHOSPHATE | non-polymer | 178.0 | 1 | Chemie (PPV) | |||
| 6 | F (A) | THYMIDINE-5'-TRIPHOSPHATE | non-polymer | 482.2 | 1 | Chemie (TTP) | |||
| 7 | G (A) | MAGNESIUM ION | non-polymer | 24.3 | 1 | Chemie (MG) | |||
| 8 | H, I, J, K (A) | SODIUM ION | non-polymer | 23.0 | 4 | Chemie (NA) | |||
| 9 | L (A) | 1,2-ETHANEDIOL | non-polymer | 62.1 | 1 | Chemie (EDO) | |||
| 10 | M (A) | S,R MESO-TARTARIC ACID | non-polymer | 150.1 | 1 | Chemie (SRT) | |||
| 11 | N, O, P, Q (A, T, P, D) | water | water | 18.0 | 242 | Chemie (HOH) |
Sequence modifications
A: 242 - 575 (UniProt: Q9UGP5)
| PDB | External Database | Details |
|---|---|---|
| Lys 463 | Ser 463 | conflict |
| Gly 464 | Gln 464 | conflict |
| - | Glu 466 | deletion |
| - | Asn 467 | deletion |
| - | Gly 468 | deletion |
| - | Gln 469 | deletion |
| - | Gln 470 | deletion |
| Thr 471 | Gln 471 | conflict |
| Ala 543 | Cys 543 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 43367.4 | |
| Non-Polymers* | Number of molecules | 9 |
| Total formula weight | 988.6 | |
| All* | Total formula weight | 44356.0 |
