7M45

DNA Polymerase Lambda, TTP:At Mg2+ Reaction State Ternary Complex, 120 sec

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2016-05-24
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1.00
Spacegroup name	P 21 21 21
Unit cell lengths	56.018, 62.547, 140.010
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	24.016 - 1.889
R-factor	0.2062
Rwork	0.204
R-free	0.24210
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3upq
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	HKL-3000
Refinement software	PHENIX (v1.15.2-3472)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.920
High resolution limit [Å]	1.889	5.130	1.890
Rmerge	0.064	0.032	0.545
Number of reflections	39892	2163	1763
<I/σ(I)>	13.1
Completeness [%]	98.9	98.1	88.5
Redundancy	11.6	12.2	4.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	277	80-95mM BICINE pH 8.3, 0.3M Na-K Tartrate, 18-22.5% PolyPure PEG