7M0D
Pre-catalytic quaternary complex of DNA Polymerase Lambda with bound complementary DSB substrate and incoming dUMPNPP
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, E (A, B) | DNA polymerase lambda | polymer | 346 | 38551.0 | 2 | UniProt (Q9UGP5) Pfam (PF14716) Pfam (PF10391) Pfam (PF14792) Pfam (PF14791) | Homo sapiens (Human) | Pol Lambda,DNA polymerase beta-2,Pol beta2,DNA polymerase kappa |
| 2 | B, F (G, K) | DNA (5'-D(*CP*GP*GP*CP*AP*GP*C)-3') | polymer | 7 | 2123.4 | 2 | synthetic construct | ||
| 3 | C, G (F, J) | DNA (5'-D(*CP*AP*GP*TP*GP*C)-3') | polymer | 6 | 1809.2 | 2 | synthetic construct | ||
| 4 | D, H (H, L) | DNA (5'-D(P*GP*CP*CP*G)-3') | polymer | 4 | 1191.8 | 2 | synthetic construct | ||
| 5 | I, J (I, E) | DNA (5'-D(*AP*CP*TP*G)-3') | polymer | 4 | 1190.8 | 2 | synthetic construct | ||
| 6 | K, Z (A, B) | 2'-DEOXYURIDINE 5'-ALPHA,BETA-IMIDO-TRIPHOSPHATE | non-polymer | 467.2 | 2 | Chemie (DUP) | |||
| 7 | AA, CA, L, N, NA (B, A, K) | SODIUM ION | non-polymer | 23.0 | 5 | Chemie (NA) | |||
| 8 | BA, M (B, A) | MAGNESIUM ION | non-polymer | 24.3 | 2 | Chemie (MG) | |||
| 9 | DA, EA, FA, O, P... (B, A) | POTASSIUM ION | non-polymer | 39.1 | 6 | Chemie (K) | |||
| 10 | HA, OA, R, S (B, J, A) | 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL | non-polymer | 122.1 | 4 | Chemie (TRS) | |||
| 11 | IA, T (B, A) | CITRIC ACID | non-polymer | 192.1 | 2 | Chemie (CIT) | |||
| 12 | JA, KA, PA, U, V... (B, J, A, F) | 1,2-ETHANEDIOL | non-polymer | 62.1 | 8 | Chemie (EDO) | |||
| 13 | GA (B) | CHLORIDE ION | non-polymer | 35.5 | 1 | Chemie (CL) | |||
| 14 | LA, MA (B) | FORMIC ACID | non-polymer | 46.0 | 2 | Chemie (FMT) | |||
| 15 | QA, RA, SA, TA, UA... (A, G, F, H, B...) | water | water | 18.0 | 1076 | Chemie (HOH) |
Sequence modifications
A, B: 234 - 575 (UniProt: Q9UGP5)
| PDB | External Database | Details |
|---|---|---|
| Gly 230 | - | expression tag |
| Ser 231 | - | expression tag |
| Ala 232 | - | expression tag |
| Ala 233 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 10 |
| Total formula weight | 89732.5 | |
| Non-Polymers* | Number of molecules | 32 |
| Total formula weight | 2829.3 | |
| All* | Total formula weight | 92561.8 |
