7LYC

Cryo-EM structure of the human nucleosome core particle ubiquitylated at histone H2A Lys13 and Lys15 in complex with BARD1 (residues 415-777)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, D (A, E)	Histone H3.1	polymer	140	15786.5	2	UniProt (P68431) Pfam (PF00125)	Homo sapiens (Human)	Histone H3/a,Histone H3/b,Histone H3/c,Histone H3/d,Histone H3/f,Histone H3/h,Histone H3/i,Histone H3/j,Histone H3/k,Histone H3/l
2	B, E (B, F)	Histone H4	polymer	107	11743.8	2	UniProt (P62805) Pfam (PF15511)	Homo sapiens (Human)
3	C, F (D, H)	Histone H2B type 1-J	polymer	126	13930.2	2	UniProt (P06899) Pfam (PF00125)	Homo sapiens (Human)	Histone H2B.1,Histone H2B.r,H2B/r
4	G (I)	DNA (147-MER)	polymer	147	45138.8	1		Homo sapiens (Human)
5	H (J)	DNA (146-MER)	polymer	147	45610.0	1		Homo sapiens (Human)
6	I (K)	Ubiquitin	polymer	84	9668.1	1	UniProt (P0CG47) Pfam (PF00240)	Homo sapiens (Human)
7	J (N)	BRCA1-associated RING domain protein 1	polymer	373	41664.6	1	UniProt (Q99728) Pfam (PF00533) Pfam (PF00023)	Homo sapiens (Human)	BARD-1,RING-type E3 ubiquitin transferase BARD1
8	K, L (C, G)	Histone H2A type 1-B/E	polymer	119	13034.2	2	UniProt (P04908) Pfam (PF00125) Pfam (PF16211)	Homo sapiens (Human)	Histone H2A.2,Histone H2A/a,Histone H2A/m

Sequence modifications

A, E: 0 - 135 (UniProt: P68431)

PDB	External Database	Details
Gly -4	-	expression tag
Pro -3	-	expression tag
Gly -2	-	expression tag
His -1	-	expression tag

B, F: 0 - 102 (UniProt: P62805)

PDB	External Database	Details
Gly -4	-	expression tag
Pro -3	-	expression tag
Gly -2	-	expression tag
His -1	-	expression tag

D, H: 0 - 123 (UniProt: P06899)

PDB	External Database	Details
Gly -2	-	expression tag
His -1	-	expression tag

K: 1 - 76 (UniProt: P0CG47)

PDB	External Database	Details
Met -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
His -1	-	expression tag
Met 0	-	expression tag

N: 415 - 777 (UniProt: Q99728)

PDB	External Database	Details
Gly 405	-	expression tag
Ser 406	-	expression tag
Gly 407	-	expression tag
Gly 408	-	expression tag
Ser 409	-	expression tag
Gly 410	-	expression tag
Gly 411	-	expression tag
Ser 412	-	expression tag
Gly 413	-	expression tag
Ser 414	-	expression tag

C, G: 12 - 129 (UniProt: P04908)

PDB	External Database	Details
Ser 11	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	12
	Total formula weight	251070.9
All*	Total formula weight	251070.9

*Water molecules are not included.