7LS5
Cryo-EM structure of the Pre3-1 20S proteasome core particle
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, O (A, O) | Proteasome subunit alpha type-1 | polymer | 252 | 28033.8 | 2 | UniProt (P21243) Pfam (PF10584) Pfam (PF00227) | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) | Macropain subunit C7-alpha,Multicatalytic endopeptidase complex C7,Proteasome component C7-alpha,Proteasome component Y8,Proteinase YSCE subunit 7,SCL1 suppressor protein |
| 2 | B, P (B, P) | Proteasome subunit alpha type-2 | polymer | 250 | 27191.8 | 2 | UniProt (P23639) Pfam (PF10584) Pfam (PF00227) | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) | Macropain subunit Y7,Multicatalytic endopeptidase complex subunit Y7,Proteasome component Y7,Proteinase YSCE subunit 7 |
| 3 | C, Q (C, Q) | Proteasome subunit alpha type-3 | polymer | 258 | 28748.2 | 2 | UniProt (P23638) Pfam (PF10584) Pfam (PF00227) | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) | Macropain subunit Y13,Multicatalytic endopeptidase complex subunit Y13,Proteasome component Y13,Proteinase YSCE subunit 13 |
| 4 | D, R (D, R) | Proteasome subunit alpha type-4 | polymer | 254 | 28478.1 | 2 | UniProt (P40303) Pfam (PF00227) Pfam (PF10584) | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) | Macropain subunit PRE6,Multicatalytic endopeptidase complex subunit PRE6,Proteasome component PRE6,Proteinase YSCE subunit PRE6 |
| 5 | E, S (E, S) | Proteasome subunit alpha type-5 | polymer | 260 | 28649.1 | 2 | UniProt (P32379) Pfam (PF10584) Pfam (PF00227) | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) | Macropain subunit PUP2,Multicatalytic endopeptidase complex subunit PUP2,Proteasome component PUP2,Proteinase YSCE subunit PUP2 |
| 6 | F, T (F, T) | Proteasome subunit alpha type-6 | polymer | 234 | 25634.0 | 2 | UniProt (P40302) Pfam (PF10584) Pfam (PF00227) | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) | Macropain subunit PRE5,Multicatalytic endopeptidase complex subunit PRE5,Proteasome component PRE5,Proteinase YSCE subunit PRE5 |
| 7 | G, U (G, U) | Proteasome subunit alpha type-7 | polymer | 288 | 31575.1 | 2 | UniProt (P21242) Pfam (PF10584) Pfam (PF00227) | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) | Macropain subunit C1,Multicatalytic endopeptidase complex subunit C1,Proteasome component C1,Proteinase YSCE subunit 1 |
| 8 | H, V (H, V) | Proteasome subunit beta type-1 | polymer | 215 | 23631.6 | 2 | UniProt (P38624) Pfam (PF00227) | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) | Macropain subunit PRE3,Multicatalytic endopeptidase complex subunit PRE3,Proteasome component PRE3,Proteinase YSCE subunit PRE3 |
| 9 | I, W (I, W) | Proteasome subunit beta type-2 | polymer | 261 | 28299.9 | 2 | UniProt (P25043) Pfam (PF00227) Pfam (PF12465) | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) | Macropain subunit PUP1,Multicatalytic endopeptidase complex subunit PUP1,Proteasome component PUP1,Proteinase YSCE subunit PUP1 |
| 10 | J, X (J, X) | Proteasome subunit beta type-3 | polymer | 205 | 22627.8 | 2 | UniProt (P25451) Pfam (PF00227) | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) | Macropain subunit PUP3,Multicatalytic endopeptidase complex subunit PUP3,Proteasome component PUP3 |
| 11 | K, Y (K, Y) | Proteasome subunit beta type-4 | polymer | 212 | 24198.5 | 2 | UniProt (P22141) Pfam (PF00227) | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) | Macropain subunit C11,Multicatalytic endopeptidase complex subunit C11,Proteasome component C11,Proteinase YSCE subunit 11 |
| 12 | L, Z (L, Z) | Proteasome subunit beta type-5 | polymer | 287 | 31670.5 | 2 | UniProt (P30656) Pfam (PF00227) | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) | Macropain subunit PRE2,Multicatalytic endopeptidase complex subunit PRE2,Proteasome component PRE2,Proteinase YSCE subunit PRE2 |
| 13 | AA, M (M, 1) | Proteasome subunit beta type-6 | polymer | 241 | 26905.1 | 2 | UniProt (P23724) Pfam (PF00227) | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) | Multicatalytic endopeptidase complex subunit C5,Proteasome component C5 |
| 14 | BA, N (N, 2) | Proteasome subunit beta type-7 | polymer | 266 | 29471.3 | 2 | UniProt (P30657) Pfam (PF00227) | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) | Macropain subunit PRE4,Multicatalytic endopeptidase complex subunit PRE4,Proteasome component PRE4,Proteinase YSCE subunit PRE4 |
Sequence modifications
H, V: -18 - 196 (UniProt: P38624)
K, Y: 0 - 197 (UniProt: P22141)
| PDB | External Database | Details |
|---|---|---|
| Asp 15 | Gly 34 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| Arg 198 | - | expression tag |
| Thr 199 | - | expression tag |
| Leu 200 | - | expression tag |
| Gln 201 | - | expression tag |
| Val 202 | - | expression tag |
| Asp 203 | - | expression tag |
| Gly 204 | - | expression tag |
| Ser 205 | - | expression tag |
| Glu 206 | - | expression tag |
| Asn 207 | - | expression tag |
| Leu 208 | - | expression tag |
| Tyr 209 | - | expression tag |
| Phe 210 | - | expression tag |
| Gln 211 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 28 |
| Total formula weight | 770229.8 | |
| All* | Total formula weight | 770229.8 |
