7LS5

Cryo-EM structure of the Pre3-1 20S proteasome core particle

Functional Information from GO Data

Chain	GOid	namespace	contents
1	0000502	cellular_component	proteasome complex
1	0003674	molecular_function	molecular_function
1	0005515	molecular_function	protein binding
1	0005634	cellular_component	nucleus
1	0005737	cellular_component	cytoplasm
1	0005829	cellular_component	cytosol
1	0005839	cellular_component	proteasome core complex
1	0010499	biological_process	proteasomal ubiquitin-independent protein catabolic process
1	0019774	cellular_component	proteasome core complex, beta-subunit complex
1	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
1	0051603	biological_process	proteolysis involved in protein catabolic process
2	0000502	cellular_component	proteasome complex
2	0003674	molecular_function	molecular_function
2	0005515	molecular_function	protein binding
2	0005634	cellular_component	nucleus
2	0005737	cellular_component	cytoplasm
2	0005829	cellular_component	cytosol
2	0005839	cellular_component	proteasome core complex
2	0010499	biological_process	proteasomal ubiquitin-independent protein catabolic process
2	0019774	cellular_component	proteasome core complex, beta-subunit complex
2	0034515	cellular_component	proteasome storage granule
2	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
2	0043248	biological_process	proteasome assembly
2	0051603	biological_process	proteolysis involved in protein catabolic process
A	0000502	cellular_component	proteasome complex
A	0003674	molecular_function	molecular_function
A	0005515	molecular_function	protein binding
A	0005634	cellular_component	nucleus
A	0005737	cellular_component	cytoplasm
A	0005739	cellular_component	mitochondrion
A	0005839	cellular_component	proteasome core complex
A	0006511	biological_process	ubiquitin-dependent protein catabolic process
A	0010499	biological_process	proteasomal ubiquitin-independent protein catabolic process
A	0019773	cellular_component	proteasome core complex, alpha-subunit complex
A	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
A	0051603	biological_process	proteolysis involved in protein catabolic process
B	0000502	cellular_component	proteasome complex
B	0003674	molecular_function	molecular_function
B	0005515	molecular_function	protein binding
B	0005634	cellular_component	nucleus
B	0005737	cellular_component	cytoplasm
B	0005839	cellular_component	proteasome core complex
B	0006511	biological_process	ubiquitin-dependent protein catabolic process
B	0010499	biological_process	proteasomal ubiquitin-independent protein catabolic process
B	0019773	cellular_component	proteasome core complex, alpha-subunit complex
B	0034515	cellular_component	proteasome storage granule
B	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
B	0051603	biological_process	proteolysis involved in protein catabolic process
C	0000502	cellular_component	proteasome complex
C	0003674	molecular_function	molecular_function
C	0005515	molecular_function	protein binding
C	0005634	cellular_component	nucleus
C	0005737	cellular_component	cytoplasm
C	0005829	cellular_component	cytosol
C	0005839	cellular_component	proteasome core complex
C	0006511	biological_process	ubiquitin-dependent protein catabolic process
C	0010499	biological_process	proteasomal ubiquitin-independent protein catabolic process
C	0019773	cellular_component	proteasome core complex, alpha-subunit complex
C	0034515	cellular_component	proteasome storage granule
C	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
C	0051603	biological_process	proteolysis involved in protein catabolic process
C	0080129	biological_process	proteasome core complex assembly
D	0000502	cellular_component	proteasome complex
D	0003674	molecular_function	molecular_function
D	0005515	molecular_function	protein binding
D	0005634	cellular_component	nucleus
D	0005737	cellular_component	cytoplasm
D	0005739	cellular_component	mitochondrion
D	0005839	cellular_component	proteasome core complex
D	0006511	biological_process	ubiquitin-dependent protein catabolic process
D	0010499	biological_process	proteasomal ubiquitin-independent protein catabolic process
D	0019773	cellular_component	proteasome core complex, alpha-subunit complex
D	0034515	cellular_component	proteasome storage granule
D	0042175	cellular_component	nuclear outer membrane-endoplasmic reticulum membrane network
D	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
D	0051603	biological_process	proteolysis involved in protein catabolic process
E	0000502	cellular_component	proteasome complex
E	0003674	molecular_function	molecular_function
E	0005515	molecular_function	protein binding
E	0005634	cellular_component	nucleus
E	0005737	cellular_component	cytoplasm
E	0005839	cellular_component	proteasome core complex
E	0006511	biological_process	ubiquitin-dependent protein catabolic process
E	0010499	biological_process	proteasomal ubiquitin-independent protein catabolic process
E	0019773	cellular_component	proteasome core complex, alpha-subunit complex
E	0034515	cellular_component	proteasome storage granule
E	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
E	0051603	biological_process	proteolysis involved in protein catabolic process
F	0000502	cellular_component	proteasome complex
F	0003674	molecular_function	molecular_function
F	0005515	molecular_function	protein binding
F	0005634	cellular_component	nucleus
F	0005737	cellular_component	cytoplasm
F	0005839	cellular_component	proteasome core complex
F	0006511	biological_process	ubiquitin-dependent protein catabolic process
F	0010499	biological_process	proteasomal ubiquitin-independent protein catabolic process
F	0019773	cellular_component	proteasome core complex, alpha-subunit complex
F	0034515	cellular_component	proteasome storage granule
F	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
F	0051603	biological_process	proteolysis involved in protein catabolic process
G	0000502	cellular_component	proteasome complex
G	0003729	molecular_function	mRNA binding
G	0005515	molecular_function	protein binding
G	0005634	cellular_component	nucleus
G	0005737	cellular_component	cytoplasm
G	0005839	cellular_component	proteasome core complex
G	0006511	biological_process	ubiquitin-dependent protein catabolic process
G	0010499	biological_process	proteasomal ubiquitin-independent protein catabolic process
G	0019773	cellular_component	proteasome core complex, alpha-subunit complex
G	0034515	cellular_component	proteasome storage granule
G	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
G	0051603	biological_process	proteolysis involved in protein catabolic process
H	0000502	cellular_component	proteasome complex
H	0004175	molecular_function	endopeptidase activity
H	0004298	molecular_function	threonine-type endopeptidase activity
H	0005515	molecular_function	protein binding
H	0005634	cellular_component	nucleus
H	0005737	cellular_component	cytoplasm
H	0005829	cellular_component	cytosol
H	0005839	cellular_component	proteasome core complex
H	0006508	biological_process	proteolysis
H	0010499	biological_process	proteasomal ubiquitin-independent protein catabolic process
H	0019774	cellular_component	proteasome core complex, beta-subunit complex
H	0034515	cellular_component	proteasome storage granule
H	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
H	0051603	biological_process	proteolysis involved in protein catabolic process
I	0000502	cellular_component	proteasome complex
I	0004175	molecular_function	endopeptidase activity
I	0004298	molecular_function	threonine-type endopeptidase activity
I	0005515	molecular_function	protein binding
I	0005634	cellular_component	nucleus
I	0005737	cellular_component	cytoplasm
I	0005829	cellular_component	cytosol
I	0005839	cellular_component	proteasome core complex
I	0006508	biological_process	proteolysis
I	0010499	biological_process	proteasomal ubiquitin-independent protein catabolic process
I	0019774	cellular_component	proteasome core complex, beta-subunit complex
I	0034515	cellular_component	proteasome storage granule
I	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
I	0051603	biological_process	proteolysis involved in protein catabolic process
J	0000502	cellular_component	proteasome complex
J	0005515	molecular_function	protein binding
J	0005634	cellular_component	nucleus
J	0005737	cellular_component	cytoplasm
J	0005829	cellular_component	cytosol
J	0005839	cellular_component	proteasome core complex
J	0010499	biological_process	proteasomal ubiquitin-independent protein catabolic process
J	0019774	cellular_component	proteasome core complex, beta-subunit complex
J	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
J	0051603	biological_process	proteolysis involved in protein catabolic process
J	0061133	molecular_function	endopeptidase activator activity
K	0000502	cellular_component	proteasome complex
K	0005515	molecular_function	protein binding
K	0005634	cellular_component	nucleus
K	0005737	cellular_component	cytoplasm
K	0005789	cellular_component	endoplasmic reticulum membrane
K	0005829	cellular_component	cytosol
K	0005839	cellular_component	proteasome core complex
K	0010498	biological_process	proteasomal protein catabolic process
K	0010499	biological_process	proteasomal ubiquitin-independent protein catabolic process
K	0019774	cellular_component	proteasome core complex, beta-subunit complex
K	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
K	0051603	biological_process	proteolysis involved in protein catabolic process
K	0061133	molecular_function	endopeptidase activator activity
L	0000502	cellular_component	proteasome complex
L	0004175	molecular_function	endopeptidase activity
L	0004298	molecular_function	threonine-type endopeptidase activity
L	0005515	molecular_function	protein binding
L	0005634	cellular_component	nucleus
L	0005737	cellular_component	cytoplasm
L	0005777	cellular_component	peroxisome
L	0005829	cellular_component	cytosol
L	0005839	cellular_component	proteasome core complex
L	0006508	biological_process	proteolysis
L	0010499	biological_process	proteasomal ubiquitin-independent protein catabolic process
L	0019774	cellular_component	proteasome core complex, beta-subunit complex
L	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
L	0051603	biological_process	proteolysis involved in protein catabolic process
L	0080129	biological_process	proteasome core complex assembly
M	0000502	cellular_component	proteasome complex
M	0003674	molecular_function	molecular_function
M	0005515	molecular_function	protein binding
M	0005634	cellular_component	nucleus
M	0005737	cellular_component	cytoplasm
M	0005829	cellular_component	cytosol
M	0005839	cellular_component	proteasome core complex
M	0010499	biological_process	proteasomal ubiquitin-independent protein catabolic process
M	0019774	cellular_component	proteasome core complex, beta-subunit complex
M	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
M	0051603	biological_process	proteolysis involved in protein catabolic process
N	0000502	cellular_component	proteasome complex
N	0003674	molecular_function	molecular_function
N	0005515	molecular_function	protein binding
N	0005634	cellular_component	nucleus
N	0005737	cellular_component	cytoplasm
N	0005829	cellular_component	cytosol
N	0005839	cellular_component	proteasome core complex
N	0010499	biological_process	proteasomal ubiquitin-independent protein catabolic process
N	0019774	cellular_component	proteasome core complex, beta-subunit complex
N	0034515	cellular_component	proteasome storage granule
N	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
N	0043248	biological_process	proteasome assembly
N	0051603	biological_process	proteolysis involved in protein catabolic process
O	0000502	cellular_component	proteasome complex
O	0003674	molecular_function	molecular_function
O	0005515	molecular_function	protein binding
O	0005634	cellular_component	nucleus
O	0005737	cellular_component	cytoplasm
O	0005739	cellular_component	mitochondrion
O	0005839	cellular_component	proteasome core complex
O	0006511	biological_process	ubiquitin-dependent protein catabolic process
O	0010499	biological_process	proteasomal ubiquitin-independent protein catabolic process
O	0019773	cellular_component	proteasome core complex, alpha-subunit complex
O	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
O	0051603	biological_process	proteolysis involved in protein catabolic process
P	0000502	cellular_component	proteasome complex
P	0003674	molecular_function	molecular_function
P	0005515	molecular_function	protein binding
P	0005634	cellular_component	nucleus
P	0005737	cellular_component	cytoplasm
P	0005839	cellular_component	proteasome core complex
P	0006511	biological_process	ubiquitin-dependent protein catabolic process
P	0010499	biological_process	proteasomal ubiquitin-independent protein catabolic process
P	0019773	cellular_component	proteasome core complex, alpha-subunit complex
P	0034515	cellular_component	proteasome storage granule
P	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
P	0051603	biological_process	proteolysis involved in protein catabolic process
Q	0000502	cellular_component	proteasome complex
Q	0003674	molecular_function	molecular_function
Q	0005515	molecular_function	protein binding
Q	0005634	cellular_component	nucleus
Q	0005737	cellular_component	cytoplasm
Q	0005829	cellular_component	cytosol
Q	0005839	cellular_component	proteasome core complex
Q	0006511	biological_process	ubiquitin-dependent protein catabolic process
Q	0010499	biological_process	proteasomal ubiquitin-independent protein catabolic process
Q	0019773	cellular_component	proteasome core complex, alpha-subunit complex
Q	0034515	cellular_component	proteasome storage granule
Q	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
Q	0051603	biological_process	proteolysis involved in protein catabolic process
Q	0080129	biological_process	proteasome core complex assembly
R	0000502	cellular_component	proteasome complex
R	0003674	molecular_function	molecular_function
R	0005515	molecular_function	protein binding
R	0005634	cellular_component	nucleus
R	0005737	cellular_component	cytoplasm
R	0005739	cellular_component	mitochondrion
R	0005839	cellular_component	proteasome core complex
R	0006511	biological_process	ubiquitin-dependent protein catabolic process
R	0010499	biological_process	proteasomal ubiquitin-independent protein catabolic process
R	0019773	cellular_component	proteasome core complex, alpha-subunit complex
R	0034515	cellular_component	proteasome storage granule
R	0042175	cellular_component	nuclear outer membrane-endoplasmic reticulum membrane network
R	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
R	0051603	biological_process	proteolysis involved in protein catabolic process
S	0000502	cellular_component	proteasome complex
S	0003674	molecular_function	molecular_function
S	0005515	molecular_function	protein binding
S	0005634	cellular_component	nucleus
S	0005737	cellular_component	cytoplasm
S	0005839	cellular_component	proteasome core complex
S	0006511	biological_process	ubiquitin-dependent protein catabolic process
S	0010499	biological_process	proteasomal ubiquitin-independent protein catabolic process
S	0019773	cellular_component	proteasome core complex, alpha-subunit complex
S	0034515	cellular_component	proteasome storage granule
S	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
S	0051603	biological_process	proteolysis involved in protein catabolic process
T	0000502	cellular_component	proteasome complex
T	0003674	molecular_function	molecular_function
T	0005515	molecular_function	protein binding
T	0005634	cellular_component	nucleus
T	0005737	cellular_component	cytoplasm
T	0005839	cellular_component	proteasome core complex
T	0006511	biological_process	ubiquitin-dependent protein catabolic process
T	0010499	biological_process	proteasomal ubiquitin-independent protein catabolic process
T	0019773	cellular_component	proteasome core complex, alpha-subunit complex
T	0034515	cellular_component	proteasome storage granule
T	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
T	0051603	biological_process	proteolysis involved in protein catabolic process
U	0000502	cellular_component	proteasome complex
U	0003729	molecular_function	mRNA binding
U	0005515	molecular_function	protein binding
U	0005634	cellular_component	nucleus
U	0005737	cellular_component	cytoplasm
U	0005839	cellular_component	proteasome core complex
U	0006511	biological_process	ubiquitin-dependent protein catabolic process
U	0010499	biological_process	proteasomal ubiquitin-independent protein catabolic process
U	0019773	cellular_component	proteasome core complex, alpha-subunit complex
U	0034515	cellular_component	proteasome storage granule
U	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
U	0051603	biological_process	proteolysis involved in protein catabolic process
V	0000502	cellular_component	proteasome complex
V	0004175	molecular_function	endopeptidase activity
V	0004298	molecular_function	threonine-type endopeptidase activity
V	0005515	molecular_function	protein binding
V	0005634	cellular_component	nucleus
V	0005737	cellular_component	cytoplasm
V	0005829	cellular_component	cytosol
V	0005839	cellular_component	proteasome core complex
V	0006508	biological_process	proteolysis
V	0010499	biological_process	proteasomal ubiquitin-independent protein catabolic process
V	0019774	cellular_component	proteasome core complex, beta-subunit complex
V	0034515	cellular_component	proteasome storage granule
V	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
V	0051603	biological_process	proteolysis involved in protein catabolic process
W	0000502	cellular_component	proteasome complex
W	0004175	molecular_function	endopeptidase activity
W	0004298	molecular_function	threonine-type endopeptidase activity
W	0005515	molecular_function	protein binding
W	0005634	cellular_component	nucleus
W	0005737	cellular_component	cytoplasm
W	0005829	cellular_component	cytosol
W	0005839	cellular_component	proteasome core complex
W	0006508	biological_process	proteolysis
W	0010499	biological_process	proteasomal ubiquitin-independent protein catabolic process
W	0019774	cellular_component	proteasome core complex, beta-subunit complex
W	0034515	cellular_component	proteasome storage granule
W	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
W	0051603	biological_process	proteolysis involved in protein catabolic process
X	0000502	cellular_component	proteasome complex
X	0005515	molecular_function	protein binding
X	0005634	cellular_component	nucleus
X	0005737	cellular_component	cytoplasm
X	0005829	cellular_component	cytosol
X	0005839	cellular_component	proteasome core complex
X	0010499	biological_process	proteasomal ubiquitin-independent protein catabolic process
X	0019774	cellular_component	proteasome core complex, beta-subunit complex
X	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
X	0051603	biological_process	proteolysis involved in protein catabolic process
X	0061133	molecular_function	endopeptidase activator activity
Y	0000502	cellular_component	proteasome complex
Y	0005515	molecular_function	protein binding
Y	0005634	cellular_component	nucleus
Y	0005737	cellular_component	cytoplasm
Y	0005789	cellular_component	endoplasmic reticulum membrane
Y	0005829	cellular_component	cytosol
Y	0005839	cellular_component	proteasome core complex
Y	0010498	biological_process	proteasomal protein catabolic process
Y	0010499	biological_process	proteasomal ubiquitin-independent protein catabolic process
Y	0019774	cellular_component	proteasome core complex, beta-subunit complex
Y	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
Y	0051603	biological_process	proteolysis involved in protein catabolic process
Y	0061133	molecular_function	endopeptidase activator activity
Z	0000502	cellular_component	proteasome complex
Z	0004175	molecular_function	endopeptidase activity
Z	0004298	molecular_function	threonine-type endopeptidase activity
Z	0005515	molecular_function	protein binding
Z	0005634	cellular_component	nucleus
Z	0005737	cellular_component	cytoplasm
Z	0005777	cellular_component	peroxisome
Z	0005829	cellular_component	cytosol
Z	0005839	cellular_component	proteasome core complex
Z	0006508	biological_process	proteolysis
Z	0010499	biological_process	proteasomal ubiquitin-independent protein catabolic process
Z	0019774	cellular_component	proteasome core complex, beta-subunit complex
Z	0043161	biological_process	proteasome-mediated ubiquitin-dependent protein catabolic process
Z	0051603	biological_process	proteolysis involved in protein catabolic process
Z	0080129	biological_process	proteasome core complex assembly

Functional Information from PROSITE/UniProt

site_id	PS00388
Number of Residues	23
Details	PROTEASOME_ALPHA_1 Proteasome alpha-type subunits signature. YdrhiTiFSPeGRlyQVEYAfkA

Chain	Residue	Details
A	TYR12-ALA34
G	TYR7-ALA29
C	TYR6-SER28
E	TYR8-ALA30
D	TYR4-ALA26
B	TYR5-ALA27
F	TYR6-ALA28

---

site_id	PS00854
Number of Residues	48
Details	PROTEASOME_BETA_1 Proteasome beta-type subunits signature. VGVkFnnGVVIAADtrstqgpivadknca.KlhrispkiwcagaGtaAD

Chain	Residue	Details
I	VAL4-ASP51
J	VAL12-ASP58
1	LEU13-ASP60
2	ILE12-ASP59
C	ILE36-ASP83
L	LEU80-ASP127
D	VAL34-ASP81
K	LEU4-ASP51

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	2
Details	ACT_SITE: Nucleophile => ECO:0000269|PubMed:9087403

Chain	Residue	Details
I	THR1
W	THR1
C	LYS199
C	LYS231
Q	LYS100
Q	LYS199
Q	LYS231

---

site_id	SWS_FT_FI2
Number of Residues	2
Details	MOD_RES: N-acetylmethionine => ECO:0007744|PubMed:22814378

Chain	Residue	Details
H	MET-18
V	MET-18
T	LYS191

---

site_id	SWS_FT_FI3
Number of Residues	2
Details	MOD_RES: Phosphoserine => ECO:0007744|PubMed:17330950, ECO:0007744|PubMed:18407956, ECO:0007744|PubMed:19779198

Chain	Residue	Details
E	SER251
S	SER251
O	LYS232

---

Catalytic Information from CSA

site_id	MCSA1
Number of Residues	8
Details	M-CSA 177

Chain	Residue	Details
I	THR1	covalently attached, hydrogen bond acceptor, hydrogen bond donor, nucleofuge, nucleophile, proton acceptor, proton donor
I	ASP17	activator, steric locator
I	ARG19	activator
I	LYS33	activator, electrostatic stabiliser
I	GLY47	electrostatic stabiliser
I	SER129	activator, electrostatic stabiliser
I	ASP166	activator, steric locator
I	SER169	activator, electrostatic stabiliser

---

site_id	MCSA2
Number of Residues	8
Details	M-CSA 177

Chain	Residue	Details
W	THR1	covalently attached, hydrogen bond acceptor, hydrogen bond donor, nucleofuge, nucleophile, proton acceptor, proton donor
W	ASP17	activator, steric locator
W	ARG19	activator
W	LYS33	activator, electrostatic stabiliser
W	GLY47	electrostatic stabiliser
W	SER129	activator, electrostatic stabiliser
W	ASP166	activator, steric locator
W	SER169	activator, electrostatic stabiliser

---