7LR6
Crystal structure of GH5_18-E140A from Bifidobacterium longum subsp. longum ATCC 55813 in complex with Manb1-4GlcNAc
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A, B, C, D | Glycosyl hydrolase BlGH5_18 | polymer | 446 | 49564.2 | 4 | UniProt (C2GY91) In PDB | Bifidobacterium longum subsp. longum ATCC 55813 | |
2 | E, G, H, I | beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 383.3 | 4 | In PDB GlyTouCan (G90333CG) | |||
3 | A, B, C, D | PHOSPHATE ION | non-polymer | 95.0 | 15 | Chemie (PO4) | |||
4 | water | water | 18.0 | 685 | Chemie (HOH) |
Sequence modifications
A, B, C, D: 1 - 426 (UniProt: C2GY91)
PDB | External Database | Details |
---|---|---|
Met -19 | - | initiating methionine |
Gly -18 | - | expression tag |
Ser -17 | - | expression tag |
Ser -16 | - | expression tag |
His -15 | - | expression tag |
His -14 | - | expression tag |
His -13 | - | expression tag |
His -12 | - | expression tag |
His -11 | - | expression tag |
His -10 | - | expression tag |
Ser -9 | - | expression tag |
Ser -8 | - | expression tag |
Gly -7 | - | expression tag |
Leu -6 | - | expression tag |
Val -5 | - | expression tag |
Pro -4 | - | expression tag |
Arg -3 | - | expression tag |
Gly -2 | - | expression tag |
Ser -1 | - | expression tag |
His 0 | - | expression tag |
Ala 140 | Glu 140 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 4 |
Total formula weight | 198256.8 | |
Branched | Number of molecules | 4 |
Total formula weight | 1533.4 | |
Non-Polymers* | Number of molecules | 15 |
Total formula weight | 1424.6 | |
All* | Total formula weight | 201214.7 |