7LR6

Crystal structure of GH5_18-E140A from Bifidobacterium longum subsp. longum ATCC 55813 in complex with Manb1-4GlcNAc

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-05-06
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.36242
Spacegroup name	P 21 21 21
Unit cell lengths	105.257, 142.989, 157.444
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.360 - 2.300
R-factor	0.1631
Rwork	0.161
R-free	0.20590
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7lr1
RMSD bond length	0.008
RMSD bond angle	0.886
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.360	2.340
High resolution limit [Å]	2.300	2.300
Number of reflections	105883	5206
<I/σ(I)>	9.1
Completeness [%]	99.9
Redundancy	13
CC(1/2)	0.996	0.533

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		292	16% PEG 2K, 0.2 M ammonium sulfate, 0.1 M sodium citrate pH 5.6