7LQW

Cryo-EM structure of NTD-directed neutralizing antibody 2-17 Fab in complex with SARS-CoV-2 S2P spike

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	H	2-17 Heavy Chain	polymer	231	24329.3	1		Homo sapiens (Human)
2	L	2-17 Light Chain	polymer	215	23441.0	1		Homo sapiens (Human)
3	B, C, A	Spike glycoprotein	polymer	1307	144106.4	3	UniProt (P0DTC2) Pfam (PF16451) Pfam (PF09408) Pfam (PF19209) Pfam (PF01601) In PDB	Severe acute respiratory syndrome coronavirus 2 (2019-nCoV SARS-CoV-2)	S glycoprotein,E2,Peplomer protein
4	A, B, C	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	26	Chemie (NAG)

Sequence modifications

B, C, A: 14 - 1208 (UniProt: P0DTC2)

PDB	External Database	Details
Met -18	-	expression tag
Gly -17	-	expression tag
Ile -16	-	expression tag
Leu -15	-	expression tag
Pro -14	-	expression tag
Ser -13	-	expression tag
Pro -12	-	expression tag
Gly -11	-	expression tag
Met -10	-	expression tag
Pro -9	-	expression tag
Ala -8	-	expression tag
Leu -7	-	expression tag
Leu -6	-	expression tag
Ser -5	-	expression tag
Leu -4	-	expression tag
Val -3	-	expression tag
Ser -2	-	expression tag
Leu -1	-	expression tag
Leu 0	-	expression tag
Ser 1	-	expression tag
Val 2	-	expression tag
Leu 3	-	expression tag
Leu 4	-	expression tag
Met 5	-	expression tag
Gly 6	-	expression tag
Cys 7	-	expression tag
Val 8	-	expression tag
Ala 9	-	expression tag
Glu 10	-	expression tag
Thr 11	-	expression tag
Gly 12	-	expression tag
Thr 13	-	expression tag
Gly 682	Arg 682	conflict
Ser 683	Arg 683	conflict
Ser 685	Arg 685	conflict
Pro 986	Lys 986	conflict
Pro 987	Val 987	conflict
Gly 1209	-	expression tag
Ser 1210	-	expression tag
Gly 1211	-	expression tag
Tyr 1212	-	expression tag
Ile 1213	-	expression tag
Pro 1214	-	expression tag
Glu 1215	-	expression tag
Ala 1216	-	expression tag
Pro 1217	-	expression tag
Arg 1218	-	expression tag
Asp 1219	-	expression tag
Gly 1220	-	expression tag
Gln 1221	-	expression tag
Ala 1222	-	expression tag
Tyr 1223	-	expression tag
Val 1224	-	expression tag
Arg 1225	-	expression tag
Lys 1226	-	expression tag
Asp 1227	-	expression tag
Gly 1228	-	expression tag
Glu 1229	-	expression tag
Trp 1230	-	expression tag
Val 1231	-	expression tag
Leu 1232	-	expression tag
Leu 1233	-	expression tag
Ser 1234	-	expression tag
Thr 1235	-	expression tag
Phe 1236	-	expression tag
Leu 1237	-	expression tag
Gly 1238	-	expression tag
Arg 1239	-	expression tag
Ser 1240	-	expression tag
Leu 1241	-	expression tag
Glu 1242	-	expression tag
Val 1243	-	expression tag
Leu 1244	-	expression tag
Phe 1245	-	expression tag
Gln 1246	-	expression tag
Gly 1247	-	expression tag
Pro 1248	-	expression tag
Gly 1249	-	expression tag
His 1250	-	expression tag
His 1251	-	expression tag
His 1252	-	expression tag
His 1253	-	expression tag
His 1254	-	expression tag
His 1255	-	expression tag
His 1256	-	expression tag
His 1257	-	expression tag
Ser 1258	-	expression tag
Ala 1259	-	expression tag
Trp 1260	-	expression tag
Ser 1261	-	expression tag
His 1262	-	expression tag
Pro 1263	-	expression tag
Gln 1264	-	expression tag
Phe 1265	-	expression tag
Glu 1266	-	expression tag
Lys 1267	-	expression tag
Gly 1268	-	expression tag
Gly 1269	-	expression tag
Gly 1270	-	expression tag
Ser 1271	-	expression tag
Gly 1272	-	expression tag
Gly 1273	-	expression tag
Gly 1274	-	expression tag
Gly 1275	-	expression tag
Ser 1276	-	expression tag
Gly 1277	-	expression tag
Gly 1278	-	expression tag
Ser 1279	-	expression tag
Ala 1280	-	expression tag
Trp 1281	-	expression tag
Ser 1282	-	expression tag
His 1283	-	expression tag
Pro 1284	-	expression tag
Gln 1285	-	expression tag
Phe 1286	-	expression tag
Glu 1287	-	expression tag
Lys 1288	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	480089.6
Non-Polymers*	Number of molecules	26
	Total formula weight	5751.4
All*	Total formula weight	485841.0

*Water molecules are not included.