7LNP

Structure of the exo-alpha-L-galactosidase BpGH29 (D264N mutant) from Bacteroides plebeius in complex with paranitrophenyl-alpha-L-galactopyranoside

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C, D, E	Exo-alpha-L-galactosidase	polymer	599	68349.3	4	UniProt (B5CYA5) Pfam (PF01120) Pfam (PF16757) In PDB	Bacteroides plebeius
2	A, C, D, E	CALCIUM ION	non-polymer		40.1	4	Chemie (CA)
3	E, A, C, D	paranitrophenyl-alpha-L-galactopyranoside	non-polymer		301.2	4	Chemie (Y9Y)
4	E, A, C, D	NICKEL (II) ION	non-polymer		58.7	9	Chemie (NI)
5	E, A, C, D	SODIUM ION	non-polymer		23.0	10	Chemie (NA)
6	E, A	CHLORIDE ION	non-polymer		35.5	2	Chemie (CL)
7		water	water		18.0	678	Chemie (HOH)

Sequence modifications

A, C, D, E: 22 - 597 (UniProt: B5CYA5)

PDB	External Database	Details
Met -1	-	initiating methionine
Gly 0	-	expression tag
Ser 1	-	expression tag
Ser 2	-	expression tag
His 3	-	expression tag
His 4	-	expression tag
His 5	-	expression tag
His 6	-	expression tag
His 7	-	expression tag
His 8	-	expression tag
Ser 9	-	expression tag
Ser 10	-	expression tag
Gly 11	-	expression tag
Leu 12	-	expression tag
Val 13	-	expression tag
Pro 14	-	expression tag
Arg 15	-	expression tag
Gly 16	-	expression tag
Ser 17	-	expression tag
His 18	-	expression tag
Met 19	-	expression tag
Ala 20	-	expression tag
Ser 21	-	expression tag
Asn 264	Asp 264	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	273397.4
Non-Polymers*	Number of molecules	29
	Total formula weight	2194.4
All*	Total formula weight	275591.7

*Water molecules are not included.