7LNP

Structure of the exo-alpha-L-galactosidase BpGH29 (D264N mutant) from Bacteroides plebeius in complex with paranitrophenyl-alpha-L-galactopyranoside

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SEALED TUBE
Source details	RIGAKU MICROMAX-003
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2014-02-11
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.5418
Spacegroup name	C 1 2 1
Unit cell lengths	157.325, 106.637, 155.896
Unit cell angles	90.00, 114.97, 90.00

Refinement procedure

Resolution	31.570 - 2.700
R-factor	0.2325
Rwork	0.230
R-free	0.28440
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7ljj
RMSD bond length	0.002
RMSD bond angle	0.419
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	32.000	2.770
High resolution limit [Å]	2.700	2.700
Rmerge	0.172	0.556
Rpim	0.119	0.396
Number of reflections	64080	4449
<I/σ(I)>	4.5	1.7
Completeness [%]	97.4
Redundancy	3.7
CC(1/2)	0.986	0.844

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	0.1 M BIS-TRIS (pH 5.5), 0.2 M (NH4)2SO4, 25% (w/v) PEG 3350