7LHZ

K. pneumoniae Topoisomerase IV (ParE-ParC) in complex with DNA and (3S)-10-[(3R)-3-(1-aminocyclopropyl)pyrrolidin-1-yl]-9-fluoro-3-methyl-5-oxo-2,3-dihydro-5H-[1,4]oxazino[2,3,4-ij]quinoline-6-carboxylic acid (compound 25)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	C, D, A, B	DNA topoisomerase 4 subunit B, DNA topoisomerase 4 subunit A chimera	polymer	743	84199.5	4	UniProt (A0A377Y395) UniProt (A0A486EJ79) Pfam (PF01751) Pfam (PF00986) Pfam (PF00521) UniProt (by SIFTS) (B5XU53) UniProt (by SIFTS) (B5XU60) In PDB	Klebsiella pneumoniae 342	Topoisomerase IV subunit B,Topoisomerase IV subunit A
2	P, J, H, E	DNA (5'-D(P*TP*AP*CP*GP*TP*TP*GP*TP*AP*T)-3')	polymer	11	3354.2	4		Klebsiella pneumoniae 342
3	K, I, G, F	DNA (5'-D(*GP*AP*TP*CP*AP*TP*AP*CP*AP*AP*CP*GP*TP*AP*A)-3')	polymer	15	4586.0	4		Klebsiella pneumoniae 342
4	C, A	(4S)-2-METHYL-2,4-PENTANEDIOL	non-polymer		118.2	2	Chemie (MPD)
5	C, D, A, B	MAGNESIUM ION	non-polymer		24.3	4	Chemie (MG)
6	K, I, G, F	(3S)-10-[(3R)-3-(1-aminocyclopropyl)pyrrolidin-1-yl]-9-fluoro-3-methyl-5-oxo-2,3-dihydro-5H-[1,4]oxazino[2,3,4-ij]quinoline-6-carboxylic acid	non-polymer		387.4	4	Chemie (Y21)
7		water	water		18.0	4	Chemie (HOH)

Sequence modifications

C, D, A, B: 390 - 998 (UniProt: A0A377Y395)

PDB	External Database	Details
Met 389	-	initiating methionine
Glu 999	-	linker
Phe 1000	-	linker

C, D, A, B: 1001 - 1490 (UniProt: A0A486EJ79)

PDB	External Database	Details
Thr 1255	Ser 255	conflict
Leu 1491	-	expression tag
Glu 1492	-	expression tag
His 1493	-	expression tag
His 1494	-	expression tag
His 1495	-	expression tag
His 1496	-	expression tag
His 1497	-	expression tag
His 1498	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	12
	Total formula weight	368558.9
Non-Polymers*	Number of molecules	10
	Total formula weight	1883.2
All*	Total formula weight	370442.1

*Water molecules are not included.