7LD4

Cryo-EM structure of the human adenosine A1 receptor-Gi2-protein complex bound to its endogenous agonist

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Guanine nucleotide-binding protein G(i) subunit alpha-2	polymer	355	40502.9	1	UniProt (P04899) Pfam (PF00503)	Homo sapiens (Human)	Adenylate cyclase-inhibiting G alpha protein
2	B (B)	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	350	38534.1	1	UniProt (P62873) Pfam (PF00400)	Homo sapiens (Human)	Transducin beta chain 1
3	C (G)	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	71	7861.1	1	UniProt (P59768) Pfam (PF00631)	Homo sapiens (Human)	G gamma-I
4	D (R)	Chimera protein of Muscarinic acetylcholine receptor M4 and Adenosine receptor A1	polymer	387	43261.0	1	UniProt (P08173) UniProt (P30542) Pfam (PF00001)	Homo sapiens (Human)
5	E (R)	ADENOSINE	non-polymer		267.2	1	Chemie (ADN)
6	F (R)	water	water		18.0	1	Chemie (HOH)

Sequence modifications

A: 1 - 355 (UniProt: P04899)

PDB	External Database	Details
Asn 47	Ser 47	conflict
Ala 204	Gly 204	conflict
Ala 246	Glu 246	conflict
Ser 327	Ala 327	conflict

B: 2 - 340 (UniProt: P62873)

PDB	External Database	Details
Met -9	-	expression tag
His -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
Ser 0	-	expression tag
Gly 1	-	expression tag

R: -28 - -7 (UniProt: P08173)

PDB	External Database	Details
Met -54	-	initiating methionine
Lys -53	-	expression tag
Thr -52	-	expression tag
Ile -51	-	expression tag
Ile -50	-	expression tag
Ala -49	-	expression tag
Leu -48	-	expression tag
Ser -47	-	expression tag
Tyr -46	-	expression tag
Ile -45	-	expression tag
Phe -44	-	expression tag
Cys -43	-	expression tag
Leu -42	-	expression tag
Val -41	-	expression tag
Phe -40	-	expression tag
Ala -39	-	expression tag
Asp -38	-	expression tag
Tyr -37	-	expression tag
Lys -36	-	expression tag
Asp -35	-	expression tag
Asp -34	-	expression tag
Asp -33	-	expression tag
Asp -32	-	expression tag
Ala -31	-	expression tag
Met -30	-	expression tag
Gly -29	-	expression tag
Leu -6	-	linker
Glu -5	-	linker
Val -4	-	linker
Leu -3	-	linker
Phe -2	-	linker
Gln -1	-	linker
Gly 0	-	linker
Pro 1	-	linker

R: 2 - 326 (UniProt: P30542)

PDB	External Database	Details
His 327	-	expression tag
His 328	-	expression tag
His 329	-	expression tag
His 330	-	expression tag
His 331	-	expression tag
His 332	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	130159.1
Non-Polymers*	Number of molecules	1
	Total formula weight	267.2
All*	Total formula weight	130426.3

*Water molecules are not included.