7LBG

CryoEM structure of the HCMV Trimer gHgLgO in complex with human Transforming growth factor beta receptor type 3 and neutralizing fabs 13H11 and MSL-109

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Envelope glycoprotein H	polymer	767	87311.3	1	UniProt (Q6SW67) Pfam (PF02489) Pfam (PF17488)	Human cytomegalovirus (strain Merlin) (HHV-5)	gH
2	B (B)	Envelope glycoprotein L	polymer	278	30846.5	1	UniProt (F5HCH8) Pfam (PF01801)	Human cytomegalovirus (strain Merlin) (HHV-5)	gL
3	C (C)	Envelope glycoprotein O	polymer	504	58298.5	1	UniProt (Q8BCU3) Pfam (PF07982)	Human cytomegalovirus (HHV-5)	UL74,UL74 protein
4	D (D)	Transforming growth factor beta receptor type 3	polymer	787	87362.2	1	UniProt (Q03167) Pfam (PF00100)	Homo sapiens (Human)	TGFR-3,Betaglycan,Transforming growth factor beta receptor III,TGF-beta receptor type III
5	E (E)	Fab 13H11 light chain	polymer	237	25780.0	1		Homo sapiens (Human)
6	F (F)	Fab 13H11 heavy chain	polymer	250	26600.1	1		Homo sapiens (Human)
7	G (G)	Fab MSL-109 light chain	polymer	257	28355.8	1		Homo sapiens (Human)
8	H (H)	Fab MSL-109 heavy chain	polymer	257	27547.8	1		Homo sapiens (Human)
9	I (I)	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		424.4	1	In PDB GlyTouCan (G42666HT)
10	J (J)	alpha-D-mannopyranose-(1-2)-alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-3)-alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		1235.1	1	In PDB GlyTouCan (G07617FP)
11	AA, K, L, M, N... (C, A, B)	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	17	Chemie (NAG)

Sequence modifications

A: 1 - 715 (UniProt: Q6SW67)

PDB	External Database	Details
Gly 716	-	expression tag
Thr 717	-	expression tag
Lys 718	-	expression tag
Leu 719	-	expression tag
Gly 720	-	expression tag
Pro 721	-	expression tag
Glu 722	-	expression tag
Gln 723	-	expression tag
Lys 724	-	expression tag
Leu 725	-	expression tag
Ile 726	-	expression tag
Ser 727	-	expression tag
Glu 728	-	expression tag
Glu 729	-	expression tag
Asp 730	-	expression tag
Leu 731	-	expression tag
Asn 732	-	expression tag
Ser 733	-	expression tag
Ala 734	-	expression tag
Val 735	-	expression tag
Asp 736	-	expression tag
Gly 737	-	expression tag
Ser 738	-	expression tag
Gly 739	-	expression tag
Leu 740	-	expression tag
Asn 741	-	expression tag
Asp 742	-	expression tag
Ile 743	-	expression tag
Phe 744	-	expression tag
Glu 745	-	expression tag
Ala 746	-	expression tag
Gln 747	-	expression tag
Lys 748	-	expression tag
Ile 749	-	expression tag
Glu 750	-	expression tag
Trp 751	-	expression tag
His 752	-	expression tag
Glu 753	-	expression tag
Asn 754	-	expression tag
Leu 755	-	expression tag
Tyr 756	-	expression tag
Phe 757	-	expression tag
Gln 758	-	expression tag
Gly 759	-	expression tag
His 760	-	expression tag
His 761	-	expression tag
His 762	-	expression tag
His 763	-	expression tag
His 764	-	expression tag
His 765	-	expression tag
His 766	-	expression tag
His 767	-	expression tag

C: 1 - 464 (UniProt: Q8BCU3)

PDB	External Database	Details
Gly 465	-	expression tag
Ser 466	-	expression tag
Glu 467	-	expression tag
Asn 468	-	expression tag
Leu 469	-	expression tag
Tyr 470	-	expression tag
Phe 471	-	expression tag
Gln 472	-	expression tag
Gly 473	-	expression tag
Ser 474	-	expression tag
Ala 475	-	expression tag
Trp 476	-	expression tag
Ser 477	-	expression tag
His 478	-	expression tag
Pro 479	-	expression tag
Gln 480	-	expression tag
Phe 481	-	expression tag
Glu 482	-	expression tag
Lys 483	-	expression tag
Gly 484	-	expression tag
Gly 485	-	expression tag
Gly 486	-	expression tag
Ser 487	-	expression tag
Gly 488	-	expression tag
Gly 489	-	expression tag
Gly 490	-	expression tag
Ser 491	-	expression tag
Gly 492	-	expression tag
Gly 493	-	expression tag
Gly 494	-	expression tag
Ser 495	-	expression tag
Ala 496	-	expression tag
Trp 497	-	expression tag
Ser 498	-	expression tag
His 499	-	expression tag
Pro 500	-	expression tag
Gln 501	-	expression tag
Phe 502	-	expression tag
Glu 503	-	expression tag
Lys 504	-	expression tag

D: 1 - 781 (UniProt: Q03167)

PDB	External Database	Details
His 782	-	expression tag
His 783	-	expression tag
His 784	-	expression tag
His 785	-	expression tag
His 786	-	expression tag
His 787	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	8
	Total formula weight	372102.2
Branched	Number of molecules	2
	Total formula weight	1659.5
Non-Polymers*	Number of molecules	17
	Total formula weight	3760.5
All*	Total formula weight	377522.2

*Water molecules are not included.