7L5M

Crystal Structure of the DiB-RM-split Protein

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Lipocalin family protein	polymer	107	12285.9	1	UniProt (A0A768MZ64) Pfam (PF08212) UniProt (by SIFTS) (P0A901)	Escherichia coli	DiB-RM-split
2	B (B)	Lipocalin family protein	polymer	69	7903.9	1	UniProt (A0A768MZ64) Pfam (PF08212) UniProt (by SIFTS) (P0A901)	Escherichia coli	DiB-RM-split
3	C (A)	2-(N-MORPHOLINO)-ETHANESULFONIC ACID	non-polymer		195.2	1	Chemie (MES)
4	D, F, G (A, B)	SULFATE ION	non-polymer		96.1	3	Chemie (SO4)
5	E (B)	SODIUM ION	non-polymer		23.0	1	Chemie (NA)
6	H, I (A, B)	water	water		18.0	12	Chemie (HOH)

Sequence modifications

A: 20 - 109 (UniProt: A0A768MZ64)

PDB	External Database	Details
Met 3	-	expression tag
Gly 4	-	expression tag
His 5	-	expression tag
His 6	-	expression tag
His 7	-	expression tag
His 8	-	expression tag
His 9	-	expression tag
His 10	-	expression tag
Leu 11	-	expression tag
Glu 12	-	expression tag
Ser 13	-	expression tag
Thr 14	-	expression tag
Ser 15	-	expression tag
Leu 16	-	expression tag
Tyr 17	-	expression tag
Lys 18	-	expression tag
Lys 19	-	expression tag
Ser 22	Pro 22	engineered mutation
Cys 36	Ala 36	engineered mutation
Ala 53	Phe 53	engineered mutation
Phe 76	Asn 76	engineered mutation
Tyr 89	Ser 89	engineered mutation
Val 90	Glu 90	engineered mutation

B: 110 - 177 (UniProt: A0A768MZ64)

PDB	External Database	Details
Met 109	-	initiating methionine
Asn 141	Leu 141	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	20189.8
Non-Polymers*	Number of molecules	5
	Total formula weight	506.4
All*	Total formula weight	20696.2

*Water molecules are not included.