7L5M

Crystal Structure of the DiB-RM-split Protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-F
Synchrotron site	APS
Beamline	21-ID-F
Temperature [K]	100
Detector technology	CCD
Collection date	2020-07-22
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.97872
Spacegroup name	P 43 21 2
Unit cell lengths	57.146, 57.146, 138.630
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.130 - 2.330
R-factor	0.2404
Rwork	0.238
R-free	0.27870
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1qwd
RMSD bond length	0.007
RMSD bond angle	1.547
Data reduction software	xia2
Data scaling software	xia2
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	46.210	46.220	2.370
High resolution limit [Å]	2.330	6.320	2.330
Rmeas	0.087	0.041	0.697
Rpim	0.030	0.015	0.286
Total number of observations	84479	4424	2781
Number of reflections	10501	623	522
<I/σ(I)>	10.9	17.8	1.8
Completeness [%]	100.0	100	100
Redundancy	8	7.1	5.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.5	294	1.6 M ammonium sulfate, 0.1 M MES, pH 4.5, supplemented with 0.5% n-Dodecyl-b-D-maltoside