7L27

Crystal structure of the catalytic domain of human PDE3A

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D (A, B, C, D)	cGMP-inhibited 3',5'-cyclic phosphodiesterase A	polymer	380	43169.1	4	UniProt (Q14432) Pfam (PF00233)	Homo sapiens (Human)	Cyclic GMP-inhibited phosphodiesterase A,CGI-PDE A,Cyclic GMP-inhibited phosphodiesterase A,CGI-PDE A,Cyclic GMP-inhibited phosphodiesterase A,CGI-PDE A
2	E, H, K, O (A, B, C, D)	MANGANESE (II) ION	non-polymer		54.9	4	Chemie (MN)
3	F, I, L, P (A, B, C, D)	MAGNESIUM ION	non-polymer		24.3	4	Chemie (MG)
4	G, J, N, Q (A, B, C, D)	ACETATE ION	non-polymer		59.0	4	Chemie (ACT)
5	M (C)	CALCIUM ION	non-polymer		40.1	1	Chemie (CA)
6	R, S, T, U (A, B, C, D)	water	water		18.0	602	Chemie (HOH)

Sequence modifications

A, B, C, D: 669 - 794 (UniProt: Q14432)

PDB	External Database	Details
Gly 668	-	expression tag

A, B, C, D: 801 - 1061 (UniProt: Q14432)

PDB	External Database	Details
Gly 795	-	linker
Gly 796	-	linker
Ser 797	-	linker
Gly 798	-	linker
Gly 799	-	linker
Ser 800	-	linker

A, B, C, D: 1068 - 1095 (UniProt: Q14432)

PDB	External Database	Details
Gly 1062	-	linker
Gly 1063	-	linker
Ser 1064	-	linker
Gly 1065	-	linker
Gly 1066	-	linker
Ser 1067	-	linker

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	172676.5
Non-Polymers*	Number of molecules	13
	Total formula weight	593.2
All*	Total formula weight	173269.7

*Water molecules are not included.