7KTK

DNA Polymerase Mu (K438D), 8-oxodGTP:Ct Ground State Ternary Complex, 50 mM Mg2+ (90min)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	DNA-directed DNA/RNA polymerase mu	polymer	356	40040.3	1	UniProt (Q9NP87) Pfam (PF14716) Pfam (PF10391) Pfam (PF14792) Pfam (PF14791) In PDB	Homo sapiens (Human)	Pol Mu,Terminal transferase
2	T	DNA (5'-D(*CP*GP*GP*CP*CP*TP*AP*CP*G)-3')	polymer	9	2716.8	1		synthetic construct
3	P	DNA (5'-D(*CP*GP*TP*A)-3')	polymer	4	1190.8	1		synthetic construct
4	D	DNA (5'-D(P*GP*CP*CP*G)-3')	polymer	4	1191.8	1		synthetic construct
5	A	8-OXO-2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE	non-polymer		523.2	1	Chemie (8DG)
6	A	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)
7	A	SODIUM ION	non-polymer		23.0	1	Chemie (NA)
8	A	4-(2-HYDROXYETHYL)-1-PIPERAZINE ETHANESULFONIC ACID	non-polymer		238.3	1	Chemie (EPE)
9	A, D	1,2-ETHANEDIOL	non-polymer		62.1	11	Chemie (EDO)
10		water	water		18.0	329	Chemie (HOH)

Sequence modifications

A: 132 - 494 (UniProt: Q9NP87)

PDB	External Database	Details
Gly 127	-	expression tag
Ser 128	-	expression tag
Ala 129	-	expression tag
Ala 130	-	expression tag
Ala 131	-	expression tag
-	Pro 398	deletion
-	Gly 399	deletion
-	Ala 400	deletion
-	Ala 401	deletion
-	Val 402	deletion
-	Gly 403	deletion
-	Gly 404	deletion
-	Ser 405	deletion
-	Thr 406	deletion
-	Arg 407	deletion
-	Pro 408	deletion
-	Cys 409	deletion
Gly 410	Pro 410	conflict
Asp 438	Lys 438	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	45139.8
Non-Polymers*	Number of molecules	16
	Total formula weight	1515.8
All*	Total formula weight	46655.6

*Water molecules are not included.