7KTK
DNA Polymerase Mu (K438D), 8-oxodGTP:Ct Ground State Ternary Complex, 50 mM Mg2+ (90min)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-12-05 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1.00 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 60.109, 61.775, 118.076 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 33.194 - 1.419 |
R-factor | 0.1441 |
Rwork | 0.143 |
R-free | 0.16840 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4m04 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.15.2_3472) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 1.440 |
High resolution limit [Å] | 1.419 | 3.850 | 1.420 |
Rmerge | 0.089 | 0.073 | 0.953 |
Rmeas | 0.098 | 0.080 | 1.054 |
Rpim | 0.039 | 0.033 | 0.444 |
Number of reflections | 83788 | 4482 | 4113 |
<I/σ(I)> | 11.4 | ||
Completeness [%] | 99.9 | 99.4 | 99.9 |
Redundancy | 6.1 | 5.8 | 5.4 |
CC(1/2) | 0.991 | 0.638 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 277 | 85-90mM HEPES pH 7.5, 17-18% PEG4000 |