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7KTK

DNA Polymerase Mu (K438D), 8-oxodGTP:Ct Ground State Ternary Complex, 50 mM Mg2+ (90min)

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsAPS BEAMLINE 22-ID
Synchrotron siteAPS
Beamline22-ID
Temperature [K]100
Detector technologyPIXEL
Collection date2018-12-05
DetectorDECTRIS EIGER X 16M
Wavelength(s)1.00
Spacegroup nameP 21 21 21
Unit cell lengths60.109, 61.775, 118.076
Unit cell angles90.00, 90.00, 90.00
Refinement procedure
Resolution33.194 - 1.419
R-factor0.1441
Rwork0.143
R-free0.16840
Structure solution methodMOLECULAR REPLACEMENT
Starting model (for MR)4m04
Data reduction softwareHKL-2000
Data scaling softwareHKL-2000
Phasing softwarePHENIX
Refinement softwarePHENIX (1.15.2_3472)
Data quality characteristics
 OverallInner shellOuter shell
Low resolution limit [Å]50.00050.0001.440
High resolution limit [Å]1.4193.8501.420
Rmerge0.0890.0730.953
Rmeas0.0980.0801.054
Rpim0.0390.0330.444
Number of reflections8378844824113
<I/σ(I)>11.4
Completeness [%]99.999.499.9
Redundancy6.15.85.4
CC(1/2)0.9910.638
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, SITTING DROP7.527785-90mM HEPES pH 7.5, 17-18% PEG4000

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