7KOX

Alpha-7 nicotinic acetylcholine receptor bound to epibatidine and PNU-120596 in the activated state

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D, E (A, B, C, D, E)	Neuronal acetylcholine receptor subunit alpha-7,Soluble cytochrome b562 fusion	polymer	562	63832.3	5	UniProt (P36544) UniProt (P0ABE7) Pfam (PF02931) Pfam (PF02932) Pfam (PF07361)	Homo sapiens (Human)	Cytochrome b-562
2	F, G, H, I, J (F, G, H, I, J)	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		424.4	5	In PDB GlyTouCan (G42666HT)
3	AA, BA, K, L, O... (E, A, B, C, D)	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	10	Chemie (NAG)
4	CA, M, Q, U, Y (E, A, B, C, D)	EPIBATIDINE	non-polymer		208.7	5	Chemie (EPJ)
5	DA, N, R, V, Z (E, A, B, C, D)	CALCIUM ION	non-polymer		40.1	5	Chemie (CA)
6	EA, FA, GA, HA, IA (A, B, C, D, E)	water	water		18.0	5	Chemie (HOH)

Sequence modifications

A, B, C, D, E: 1 - 321 (UniProt: P36544)
A, B, C, D, E: 321 - 325 (UniProt: P0ABE7)

PDB	External Database	Details
Trp 321	Met 29	variant
Ile 325	His 124	variant
Leu 325	Arg 128	variant

A, B, C, D, E: 325 - 479 (UniProt: P36544)

PDB	External Database	Details
Ala 321	-	linker
Gly 321	-	linker
Ala 321	-	linker
Met 321	-	linker
Trp 480	-	expression tag
Ser 481	-	expression tag
His 482	-	expression tag
Pro 483	-	expression tag
Gln 484	-	expression tag
Phe 485	-	expression tag
Glu 486	-	expression tag
Lys 487	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	319161.5
Branched	Number of molecules	5
	Total formula weight	2122.0
Non-Polymers*	Number of molecules	20
	Total formula weight	3455.9
All*	Total formula weight	324739.4

*Water molecules are not included.