7KOA

Room Temperature Structure of SARS-CoV-2 Nsp10/16 Methyltransferase in a Complex with Cap-0 and SAM Determined by Pink-Beam Serial Crystallography

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	2'-O-methyltransferase	polymer	301	33627.5	1	UniProt (P0DTD1) Pfam (PF06460) In PDB	Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)	Non-structural protein 16
2	B	Non-structural protein 10	polymer	142	15075.2	1	UniProt (P0DTD1) Pfam (PF09401) In PDB	Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)
3	A	P1-7-METHYLGUANOSINE-P3-ADENOSINE-5',5'-TRIPHOSPHATE	non-polymer		787.4	2	Chemie (GTA)
4	A	S-ADENOSYLMETHIONINE	non-polymer		398.4	1	Chemie (SAM)
5	B	ZINC ION	non-polymer		65.4	2	Chemie (ZN)
6		water	water		18.0	182	Chemie (HOH)

Sequence modifications

A: 1 - 298 (UniProt: P0DTD1)

PDB	External Database	Details
Ser -2	-	expression tag
Asn -1	-	expression tag
Ala 0	-	expression tag

B: 1 - 139 (UniProt: P0DTD1)

PDB	External Database	Details
Ser -2	-	expression tag
Asn -1	-	expression tag
Ala 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	48702.7
Non-Polymers*	Number of molecules	5
	Total formula weight	2104.1
All*	Total formula weight	50806.8

*Water molecules are not included.