7KO3

Dihydrodipicolinate synthase (DHDPS) from C.jejuni, E88D mutant with pyruvate bound in the active site and L-lysine bound at the allosteric site

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D, E... (A, B, C, D, E...)	4-hydroxy-tetrahydrodipicolinate synthase	polymer	310	34171.2	6	UniProt (Q9PPB4) Pfam (PF00701)	Campylobacter jejuni subsp. jejuni serotype O:2 (strain ATCC 700819 / NCTC 11168)	HTPA synthase
2	BA, G, M, R, U... (F, A, B, C, D...)	LYSINE	non-polymer		147.2	6	Chemie (LYS)
3	CA, H, I, J, N... (F, A, B, D)	MAGNESIUM ION	non-polymer		24.3	7	Chemie (MG)
4	K, L, T, W, Z (A, C, D, E)	TRIETHYLENE GLYCOL	non-polymer		150.2	5	Chemie (PGE)
5	AA, EA, P, X (E, F, B, D)	1,2-ETHANEDIOL	non-polymer		62.1	4	Chemie (EDO)
6	FA, Q (F, B)	DI(HYDROXYETHYL)ETHER	non-polymer		106.1	2	Chemie (PEG)
7	DA, S (F, C)	ACETATE ION	non-polymer		59.0	2	Chemie (ACT)
8	GA, HA, IA, JA, KA... (A, B, C, D, E...)	water	water		18.0	745	Chemie (HOH)

Sequence modifications

A, B, C, D, E, F: 1 - 298 (UniProt: Q9PPB4)

PDB	External Database	Details
Met -11	-	expression tag
Arg -10	-	expression tag
Gly -9	-	expression tag
Ser -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
Gly -1	-	expression tag
Ser 0	-	expression tag
Asp 88	Glu 88	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	205027.4
Non-Polymers*	Number of molecules	26
	Total formula weight	2382.8
All*	Total formula weight	207410.2

*Water molecules are not included.