7KL3

The crystal structure of the 2009/H1N1/California PA endonuclease mutant E119D bound to RNA oligomer AG*CAUC (*uncleaveable bond, -UC disordered)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Protein PA-X	polymer	197	23134.3	1	UniProt (A0A4P2TE19) UniProt (A0A646SQW7) UniProt (by SIFTS) (A0A5J6VBC3)	Influenza A virus
2	B (B)	RNA (5'-R(P*AP*GP*CP*A)-3')	polymer	6	1875.2	1		synthetic construct
3	C (A)	GLYCEROL	non-polymer		92.1	1	Chemie (GOL)
4	D, E (A)	SULFATE ION	non-polymer		96.1	2	Chemie (SO4)
5	F (B)	ADENOSINE MONOPHOSPHATE	non-polymer		347.2	1	Chemie (AMP)
6	G (B)	5'-O-sulfocytidine	non-polymer		323.3	1	Chemie (RQA)
7	H, I (A, B)	water	water		18.0	120	Chemie (HOH)

Sequence modifications

A: 1 - 50 (UniProt: A0A4P2TE19)

PDB	External Database	Details
Met -19	-	expression tag
Gly -18	-	expression tag
Ser -17	-	expression tag
Ser -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
Ser -9	-	expression tag
Ser -8	-	expression tag
Gly -7	-	expression tag
Leu -6	-	expression tag
Val -5	-	expression tag
Pro -4	-	expression tag
Arg -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag
Gly 51	-	linker
Gly 52	-	linker
Ser 53	-	linker

A: 73 - 196 (UniProt: A0A646SQW7)

PDB	External Database	Details
Ile 85	Thr 85	conflict
Asp 119	Glu 119	engineered mutation
Ser 186	Gly 186	conflict

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	25009.5
Non-Polymers*	Number of molecules	5
	Total formula weight	954.7
All*	Total formula weight	25964.2

*Water molecules are not included.