7KL3
The crystal structure of the 2009/H1N1/California PA endonuclease mutant E119D bound to RNA oligomer AG*CAUC (*uncleaveable bond, -UC disordered)
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Protein PA-X | polymer | 197 | 23134.3 | 1 | UniProt (A0A4P2TE19) UniProt (A0A646SQW7) UniProt (by SIFTS) (A0A5J6VBC3) | Influenza A virus | |
| 2 | B (B) | RNA (5'-R(P*AP*GP*CP*A)-3') | polymer | 6 | 1875.2 | 1 | synthetic construct | ||
| 3 | C (A) | GLYCEROL | non-polymer | 92.1 | 1 | Chemie (GOL) PubChem (753) | |||
| 4 | D, E (A) | SULFATE ION | non-polymer | 96.1 | 2 | Chemie (SO4) | |||
| 5 | F (B) | ADENOSINE MONOPHOSPHATE | non-polymer | 347.2 | 1 | Chemie (AMP) PubChem (16397049) PubChem (16760266) PubChem (224) PubChem (40467049) PubChem (40469468) PubChem (34768) PubChem (44348067) PubChem (83862) PubChem (45358029) PubChem (46874558) PubChem (40459109) PubChem (57358045) PubChem (57879893) PubChem (57879902) PubChem (46875232) PubChem (446048) PubChem (515384) PubChem (638042) PubChem (177678969) PubChem (6083) PubChem (1549371) PubChem (5316444) PubChem (92144436) PubChem (5742864) PubChem (122197642) PubChem (12358354) PubChem (12163610) PubChem (12358355) PubChem (162290139) PubChem (162946266) PubChem (171699505) | |||
| 6 | G (B) | 5'-O-sulfocytidine | non-polymer | 323.3 | 1 | Chemie (RQA) PubChem (20511733) PubChem (22814079) | |||
| 7 | H, I (A, B) | water | water | 18.0 | 120 | Chemie (HOH) |
Sequence modifications
A: 1 - 50 (UniProt: A0A4P2TE19)
A: 73 - 196 (UniProt: A0A646SQW7)
| PDB | External Database | Details |
|---|---|---|
| Met -19 | - | expression tag |
| Gly -18 | - | expression tag |
| Ser -17 | - | expression tag |
| Ser -16 | - | expression tag |
| His -15 | - | expression tag |
| His -14 | - | expression tag |
| His -13 | - | expression tag |
| His -12 | - | expression tag |
| His -11 | - | expression tag |
| His -10 | - | expression tag |
| Ser -9 | - | expression tag |
| Ser -8 | - | expression tag |
| Gly -7 | - | expression tag |
| Leu -6 | - | expression tag |
| Val -5 | - | expression tag |
| Pro -4 | - | expression tag |
| Arg -3 | - | expression tag |
| Gly -2 | - | expression tag |
| Ser -1 | - | expression tag |
| His 0 | - | expression tag |
| Gly 51 | - | linker |
| Gly 52 | - | linker |
| Ser 53 | - | linker |
| PDB | External Database | Details |
|---|---|---|
| Ile 85 | Thr 85 | conflict |
| Asp 119 | Glu 119 | engineered mutation |
| Ser 186 | Gly 186 | conflict |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 25009.5 | |
| Non-Polymers* | Number of molecules | 5 |
| Total formula weight | 954.7 | |
| All* | Total formula weight | 25964.2 |
