7KL3
The crystal structure of the 2009/H1N1/California PA endonuclease mutant E119D bound to RNA oligomer AG*CAUC (*uncleaveable bond, -UC disordered)
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A | Protein PA-X | polymer | 197 | 23134.3 | 1 | UniProt (A0A4P2TE19) UniProt (A0A646SQW7) Pfam (PF00603) UniProt (by SIFTS) (A0A5J6VBC3) In PDB | Influenza A virus | |
2 | B | RNA (5'-R(P*AP*GP*CP*A)-3') | polymer | 6 | 1875.2 | 1 | synthetic construct | ||
3 | A | GLYCEROL | non-polymer | 92.1 | 1 | Chemie (GOL) | |||
4 | A | SULFATE ION | non-polymer | 96.1 | 2 | Chemie (SO4) | |||
5 | B | ADENOSINE MONOPHOSPHATE | non-polymer | 347.2 | 1 | Chemie (AMP) | |||
6 | B | 5'-O-sulfocytidine | non-polymer | 323.3 | 1 | Chemie (RQA) | |||
7 | water | water | 18.0 | 120 | Chemie (HOH) |
Sequence modifications
A: 1 - 50 (UniProt: A0A4P2TE19)
A: 73 - 196 (UniProt: A0A646SQW7)
PDB | External Database | Details |
---|---|---|
Met -19 | - | expression tag |
Gly -18 | - | expression tag |
Ser -17 | - | expression tag |
Ser -16 | - | expression tag |
His -15 | - | expression tag |
His -14 | - | expression tag |
His -13 | - | expression tag |
His -12 | - | expression tag |
His -11 | - | expression tag |
His -10 | - | expression tag |
Ser -9 | - | expression tag |
Ser -8 | - | expression tag |
Gly -7 | - | expression tag |
Leu -6 | - | expression tag |
Val -5 | - | expression tag |
Pro -4 | - | expression tag |
Arg -3 | - | expression tag |
Gly -2 | - | expression tag |
Ser -1 | - | expression tag |
His 0 | - | expression tag |
Gly 51 | - | linker |
Gly 52 | - | linker |
Ser 53 | - | linker |
PDB | External Database | Details |
---|---|---|
Ile 85 | Thr 85 | conflict |
Asp 119 | Glu 119 | engineered mutation |
Ser 186 | Gly 186 | conflict |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 2 |
Total formula weight | 25009.5 | |
Non-Polymers* | Number of molecules | 5 |
Total formula weight | 954.7 | |
All* | Total formula weight | 25964.2 |