7KJR

Cryo-EM structure of SARS-CoV-2 ORF3a

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	ORF3a protein	polymer	284	32165.9	2	UniProt (P0DTC3) Pfam (PF11289)	Severe acute respiratory syndrome coronavirus 2 (2019-nCoV)	Accessory protein 3a,Protein U274,Protein X1,ORF3a,Protein 3a
2	C, D (C, D)	Apolipoprotein A-I	polymer	210	24647.7	2	UniProt (P02647) Pfam (PF01442)	Homo sapiens (Human)	ApoA-I,Apolipoprotein A1,MSPE3D1
3	E, F (A, B)	1,2-dioleoyl-sn-glycero-3-phosphoethanolamine	non-polymer		744.0	2	Chemie (PEE)
4	G, H (A, B)	water	water		18.0	122	Chemie (HOH)

Sequence modifications

A, B: 1 - 275 (UniProt: P0DTC3)

PDB	External Database	Details
Ser 276	-	expression tag
Asn 277	-	expression tag
Ser 278	-	expression tag
Leu 279	-	expression tag
Glu 280	-	expression tag
Val 281	-	expression tag
Leu 282	-	expression tag
Phe 283	-	expression tag
Gln 284	-	expression tag

C, D: 23 - 211 (UniProt: P02647)

PDB	External Database	Details
His 2	-	expression tag
His 3	-	expression tag
His 4	-	expression tag
His 5	-	expression tag
His 6	-	expression tag
His 7	-	expression tag
His 8	-	expression tag
Asp 9	-	expression tag
Tyr 10	-	expression tag
Asp 11	-	expression tag
Ile 12	-	expression tag
Pro 13	-	expression tag
Thr 14	-	expression tag
Thr 15	-	expression tag
Glu 16	-	expression tag
Asn 17	-	expression tag
Leu 18	-	expression tag
Tyr 19	-	expression tag
Phe 20	-	expression tag
Gln 21	-	expression tag
Gly 22	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	113627.2
Non-Polymers*	Number of molecules	2
	Total formula weight	1488.1
All*	Total formula weight	115115.2

*Water molecules are not included.