7KH6
1.45 Angstrom resolution internal aldimine crystal structure of the beta-Q114A mutant of TryptophanSynthase in complex with N-(4'-trifluoromethoxybenzenesulfonyl)-2-amino-1-ethylphosphate (F9F) at the enzyme alpha-site and cesium ion at the metal coordination site
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Tryptophan synthase alpha chain | polymer | 268 | 28698.8 | 1 | UniProt (P00929) Pfam (PF00290) | Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) | |
| 2 | B (B) | Tryptophan synthase beta chain | polymer | 397 | 42861.8 | 1 | UniProt (P0A2K1) Pfam (PF00291) | Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) | |
| 3 | C, D, E, F, G... (A, B) | DIMETHYL SULFOXIDE | non-polymer | 78.1 | 13 | Chemie (DMS) | |||
| 4 | J (A) | 2-({[4-(TRIFLUOROMETHOXY)PHENYL]SULFONYL}AMINO)ETHYL DIHYDROGEN PHOSPHATE | non-polymer | 365.2 | 1 | Chemie (F9F) | |||
| 5 | K, U, V, W (A, B) | 1,2-ETHANEDIOL | non-polymer | 62.1 | 4 | Chemie (EDO) | |||
| 6 | AA, BA, L (B, A) | CHLORIDE ION | non-polymer | 35.5 | 3 | Chemie (CL) | |||
| 7 | M (B) | DI(HYDROXYETHYL)ETHER | non-polymer | 106.1 | 1 | Chemie (PEG) | |||
| 8 | N (B) | PYRIDOXAL-5'-PHOSPHATE | non-polymer | 247.1 | 1 | Chemie (PLP) | |||
| 9 | X, Y, Z (B) | CESIUM ION | non-polymer | 132.9 | 3 | Chemie (CS) | |||
| 10 | CA, DA (A, B) | water | water | 18.0 | 811 | Chemie (HOH) |
Sequence modifications
B: 1 - 397 (UniProt: P0A2K1)
| PDB | External Database | Details |
|---|---|---|
| Ala 114 | Gln 114 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 71560.6 | |
| Non-Polymers* | Number of molecules | 26 |
| Total formula weight | 2487.6 | |
| All* | Total formula weight | 74048.2 |
