7KH4

Dihydrodipicolinate synthase (DHDPS) from C.jejuni, H56W mutant with pyruvate bound in the active site and R,R-bislysine bound at the allosteric site

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D, E... (A, B, C, D, E...)	4-hydroxy-tetrahydrodipicolinate synthase	polymer	310	34233.3	6	UniProt (Q9PPB4) Pfam (PF00701)	Campylobacter jejuni subsp. jejuni serotype O:2 (strain ATCC 700819 / NCTC 11168)	HTPA synthase
2	AA, BB, G (B, D, A)	(2R,5R)-2,5-diamino-2,5-bis(4-aminobutyl)hexanedioic acid	non-polymer		318.4	3	Chemie (3VN)
3	BA, CA, CB, DA, EC... (B, D, F, A, E...)	MAGNESIUM ION	non-polymer		24.3	19	Chemie (MG)
4	DB, EA, IC, L, SA... (D, B, F, A, C...)	TETRAETHYLENE GLYCOL	non-polymer		194.2	6	Chemie (PG4)
5	AC, EB, FA, FB, GA... (E, D, B, F, A...)	1,2-ETHANEDIOL	non-polymer		62.1	33	Chemie (EDO)
6	BC, IB, KA, LA, LC... (E, D, B, F, A...)	DI(HYDROXYETHYL)ETHER	non-polymer		106.1	10	Chemie (PEG)
7	CC, JB, KB, LB, MB... (E, D, B, F, A)	ACETATE ION	non-polymer		59.0	13	Chemie (ACT)
8	DC, NB, OB, PA (E, D, B)	TRIETHYLENE GLYCOL	non-polymer		150.2	4	Chemie (PGE)
9	AB (C)	GLYCEROL	non-polymer		92.1	1	Chemie (GOL)
10	RC, SC, TC, UC, VC... (A, B, C, D, E...)	water	water		18.0	1116	Chemie (HOH)

Sequence modifications

A, B, C, D, E, F: 1 - 298 (UniProt: Q9PPB4)

PDB	External Database	Details
Met -11	-	expression tag
Arg -10	-	expression tag
Gly -9	-	expression tag
Ser -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
Gly -1	-	expression tag
Ser 0	-	expression tag
Trp 56	His 56	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	205399.9
Non-Polymers*	Number of molecules	89
	Total formula weight	7152.2
All*	Total formula weight	212552.1

*Water molecules are not included.