7KH0

Cryo-EM structure of the human arginine vasopressin AVP-vasopressin receptor V2R-Gs signaling complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (R)	Vasopressin V2 receptor	polymer	389	42346.1	1	UniProt (P30518) Pfam (PF00001)	Homo sapiens (Human)	V2R,AVPR V2,Antidiuretic hormone receptor,Renal-type arginine vasopressin receptor
2	B (B)	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	350	38613.2	1	UniProt (P62873) Pfam (PF00400)	Homo sapiens (Human)	Transducin beta chain 1
3	C (A)	Guanine nucleotide-binding protein G(i) subunit alpha-3, Isoform Gnas-2 of Guanine nucleotide-binding protein G(s) subunit alpha isoforms short fusion	polymer	372	43298.1	1	UniProt (P08754) UniProt (P63092-2) Pfam (PF00503) UniProt (by SIFTS) (P63092)	Homo sapiens (Human)	G(i) alpha-3,Adenylate cyclase-stimulating G alpha protein
4	D (L)	Arg-vasopressin	polymer	10	1086.2	1	UniProt (P01185) Pfam (PF00220)	Homo sapiens (Human)	AVP-NPII
5	E (G)	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	67	7432.6	1	UniProt (P59768) Pfam (PF00631)	Homo sapiens (Human)	G gamma-I
6	F (S)	Single Fab chain (scFv16)	polymer	248	26450.5	1		Homo sapiens (Human)
7	G (N)	Nanobody 35	polymer	134	14714.3	1		Lama glama (llama)

Sequence modifications

R: 1 - 371 (UniProt: P30518)

PDB	External Database	Details
Asp -8	-	expression tag
Tyr -7	-	expression tag
Lys -6	-	expression tag
Asp -5	-	expression tag
Asp -4	-	expression tag
Asp -3	-	expression tag
Asp -2	-	expression tag
Val -1	-	expression tag
Asp 0	-	expression tag
Ala 372	-	expression tag
Ala 373	-	expression tag
Ala 374	-	expression tag
Leu 375	-	expression tag
Glu 376	-	expression tag
Val 377	-	expression tag
Leu 378	-	expression tag
Phe 379	-	expression tag
Gln 380	-	expression tag

B: 2 - 340 (UniProt: P62873)

PDB	External Database	Details
His -9	-	expression tag
His -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
Gly -3	-	expression tag
Ser -2	-	expression tag
Leu -1	-	expression tag
Leu 0	-	expression tag
Gln 1	-	expression tag

A: 9 - 25 (UniProt: P08754)
A: 26 - 394 (UniProt: P63092-2)

PDB	External Database	Details
Asp 188	Ala 174	conflict

L: 1 - 9 (UniProt: P01185)

PDB	External Database	Details
Nh2 10	-	amidation

Contents of the asymmetric unit

Polymers	Number of chains	7
	Total formula weight	173941.0
All*	Total formula weight	173941.0

*Water molecules are not included.